Click image to see more details
Product Info Summary
| SKU: | A06845-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-ENOB Antibody (Center)
SKU/Catalog Number
A06845-2
Size
80 µl
Description
Boster Bio Anti-ENOB Antibody (Center) (Catalog # A06845-2). Tested in WB, IHC-P, Flow Cytometry application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ENOB Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06845-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ENOB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-264 amino acids from the Central region of human ENOB.
Reactive Species
A06845-2 is reactive to ENO3 in Human, Mouse, Rat
Calculated molecular weight
47.0 kDa
Background of ENO3
ENOB is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in its gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06845-2 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
FC: 1:10-1:50
Validation Images & Assay Conditions
Click image to see more details
ENOB Antibody (Center) western blot analysis in RD cell line and mouse skeletal muscle,rat skeletal muscle lysates (35ug/lane).This demonstrates the ENOB antibody detected the ENOB protein (arrow).
Click image to see more details
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ENOB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Click image to see more details
ENOB Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-ENOB Antibody (Center) (A06845-2)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-ENOB Antibody (Center)?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-ENOB Antibody (Center)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
