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- Table of Contents
FASN · Western blot design guide
FASN Western Blot Planning Guide
Plan a FASN Western blot around the catalog-observed 273.4 kDa band, image-backed PB9865 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
FASN Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 273.4 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 4-12% gradient | |
| Positive control | Adipose tissue | |
| Negative control | Appendix |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 273.4 kDa |
| ⓘ | Localization | Cytoplasm / Melanosome |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Sample controls for FASN Western blot
🧪Use Adipose tissue as the first positive-control candidate and Appendix as the HPA Not detected negative candidate.
Positive control: Adipose tissue (High)
Negative control: Appendix (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for FASN
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.
Section 1
What Is the Expected FASN Western Blot Band Size?
Use the product-observed 273.4 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 273.4 kDa | Matches the authoritative product WB observation. |
| 273.4 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected FASN appearancePlan around 273.4 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 273.4 kDa; use this as the primary experimental expectation. |
| Calculated mass | 273.4 kDa from UniProt P49327; retain as context. |
| Gel selection | 4-12% gradient; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PB9865. |
| Situation | Likely cause | Next action |
|---|---|---|
| 273.4 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing FASN band
PB9865 · FASNObserved 273.4 kDa · product-specific WB image
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Section 2
Real Curated FASN Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Adipose tissue |
| Gel % | 4-12% gradient |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PB9865 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 4
Advanced FASN Western Blot Tips
Deeper troubleshooting and optimisation questions for FASN, answered from its protein features.
Which band should guide the blot?
Use 273.4 kDa, the observation attached to the authoritative PB9865 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 273.4 kDa expectation.
Which positive control should I start with?
Start with Adipose tissue, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Appendix as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 4-12% gradient consistently across the quick facts, protocol table, and poster.
What transfer method to use for FASN Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PB9865 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
Compare only the supplied target-verified records: PMC8329134, PMC12084808, PMC8530891.
Boster reagents
FASN Western Blot Reagents
Human/Mouse/Rat-reactive FASN Western blot reagents with authoritative product imagery.
Real WB data
Anti-Fatty Acid Synthase/FASN Antibody Picoband®
Cat # PB9865
Reactivity: Human, Mouse, Rat
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P49327
- Human Protein Atlas — FASN
- PB9865 product record
- PMC8329134 — Reducing FASN expression sensitizes acute myeloid leukemia cells to differentiation therapy.
- PMC12084808 — ERRATUM: Roles of the HOXA10 gene during castrate-resistant prostate cancer progression.
- PMC8530891 — Fatty acid synthase mediates high glucose-induced EGFR activation in oral dysplastic keratinocytes.