This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Facts about Peptidyl-prolyl cis-trans isomerase FKBP4.
May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening.
Human | |
---|---|
Gene Name: | FKBP4 |
Uniprot: | Q02790 |
Entrez: | 2288 |
Belongs to: |
---|
No superfamily |
51 kDa FK506-binding protein; 52 kDa FKBP; 59 kDa immunophilin; EC 5.2.1.8; FK506 binding protein 4, 59kDa; FK506-binding protein 4 (59kD); FK506-binding protein 4; FKBP4; FKBP-4; FKBP51; FKBP52; FKBP-52; FKBP52T-cell FK506-binding protein, 59kD; FKBP59; FKBP5952 kDa FK506-binding protein; HBI; HSP binding immunophilin; Hsp56; HSP-binding immunophilin; Immunophilin FKBP52; p52; p59; peptidyl-prolyl cis-trans isomerase FKBP4; peptidylprolyl cis-trans isomerase; PPIase FKBP4; PPIase; rotamase
Mass (kDA):
51.805 kDA
Human | |
---|---|
Location: | 12p13.33 |
Sequence: | 12; NC_000012.12 (2794970..2805423) |
Widely expressed.
Cytoplasm, cytosol. Mitochondrion. Nucleus. Cytoplasm, cytoskeleton. Cell projection, axon. Shuttles from mitochondria to nucleus; co-localizes in mitochondria with the glucocorticoid receptor (PubMed:21730050). Colocalized with MAPT/TAU in the distal part of the primary cortical neurons (By similarity).
Boster Bio Anti FKBP4 has been tested using ICC, IHC and Flow Cytometry. The product reacts well with FKBP52 and Human. It is useful for many purposes. Boster Bio's Anti-FKBP4 antibody is available for purchase. This article will cover some of these benefits.
Boster Bio Anti-FKBP52 is the best anti-FKBP4 marker antibody. This monoclonal antibody is a high affinity product tested for use in immunohistochemistry, ICC and Western Blotting. It reacts to both Human and Mouse cell. This antibody can also used for IHC analysis. Boster Bio's Anti-FKBP52 antibody is a valuable tool for your research.
The 52-kDa FKBP52 genetic is involved in signaling at steroid hormones receptors. Its expression was investigated in breast cancer. The gene expression was determined using immunohistochemistry in 145 breast cancer samples and 66 non-cancerous tissues as controls. Kaplan Meier survival analysis was done on the data. The FKBP52 levels were moderately higher in breast cancers than in noncancerous tissues.
In many biological assays, FKBP4 is detected with an antibody. The antibodies react with most animal samples and can be monoclonal, polyclonal, or polyclonal. Boster Bio developed its antibodies to the FKBP4 protein by using rabbits and mice. This antibody has been shown to interact with heat-shock protein 90. It may also play part in intracellular trafficking steroid hormone receptors. Its isomerase activity regulates opening TRPC1 channels in neuronal grow cones.
Boster Bio's successful testing of Anti-FKBP52 Boster Bio used IP, IF, IHC and WB assays. This antibody has been validated against positive and negative samples. If you are unsure about the sensitivity to FKBP+ cells, consult a physician. Anti-FKBP52 Boster Bio gives a complete guide on anti FKBP4 antibody.
This antibody has been enhanced by a high-sensitivity ELISA panel. This antibody is validated with a wide range samples and comes with a validation protocol. Supervision is a polymer-based secondary antibody that can save you up to 30 minutes of IHC. This antibody also comes with technical support from Sanbio distributor BeNeLux. You can also download a validation protocol and images for reference.
Primary antibodies are immunoglobulins which bind to the antigen they were made to recognize. High-affinity antibodies are also known for their specificity. The quality and strength of the noncovalent link between the primary antibody (and the target protein) determines the quality. High-affinity primary antibody can detect, purify or measure the antigens they recognize.
The FKBP4 antibody is supplied in a buffer which contains 10 mM sodium HEPES, 150 mM NACl, and 100 Ug/ml BSA. The antibody does not cross-react to other FKBP proteins. A simple 1:1 fitting model was used for plotting steady-state binding levels against the analyte level to calculate FKBP4's KD. These experiments were compatible with Biacore Evaluation Software. FKBP4's KD values were calculated as 192 +41 uM. These correspond to the low affinity of the (ATP]Hsp70/aSyn-complex.
A Boster Bio FKBP4 marker for the high-affinity primary antibody is a highly effective tool for detecting specific antigens. Researchers can use the same primary antibody to do dual-labeling tests. Dual-labeling capabilities allow researchers to ask greater questions and gather more context data. These tests have proven to be valuable tools for scientists and researchers alike.
The aSyn antibody is highly purified. The antigen is then incubated together with Hsp70 using a buffer containing 50 M Tris pH 7.4 & 2 mM mM GlyCl2. The antigen-antibody mixture is incubated with four mM of ATP for two hours. The resulting complex is high in affinity if the Hsp70 antibody and aSyn have met these requirements.
Antibodies to FKBP4 are widely used for biochemical and molecular biology applications. They can be purchased as monoclonal, polyclonal antibodies and can react with FKBP4 from many animal samples. Boster Bio uses rabbit and mouse as a model organism to develop FKBP4 antibodies. This immunophilin proteins has both co-chaperone and PPIase activities. It may be involved in intracellular trafficking and regulation of heterooligomeric steroidhormone receptors. Its isomerase activity regulates TRPC1 channel open and controls neuronal-growth cones.
FKBP4 (a marker for cancer) is used to determine whether a patient's likelihood of developing the disease. FKBP4 is also implicated in LUAD progression. In animal models, it contributes to the growth of tumor cells and metastasis. It can also be used in the development of new therapeutic strategies. However, despite the benefits, this marker has a long way to go before it can become a clinically useful marker.
This marker is made in h2975 cells. It interacts to two proteins, RelA (or Hsp70). Both h2975 cells and BEAS-2B cells have similar gene expression profiles and produce endogenous FKBP4. The results of the LUAD cells were consistent with a previous study in 293T cells. LUAD cells also contain a high level of FKBP4.
GenePharma, BioSune and BioSune designed siRNAs targeting the FKBP4 marker. They used plasmids that contained human Hsp90 and IKKb as well as RelA. They used Lipofectamine 3000 to transfect. FKBP4 was also produced. These markers were later used in transfections as a marker for cancer progression prediction.
The FKBP4/NR3C1/NRF2 axis plays a pivotal role in regulating naringenin-restrained cell proliferation. T47D cells lacking FKBP4 showed lower cell viability in comparison to cells that were overexpressing NR3C1. TNFa or GA could be used to knockdown FKBP4 and increase cell viability in T47D or BT549, respectively.
PMID: 1279700 by Peattie D.A., et al. Expression and characterization of human FKBP52, an immunophilin that associates with the 90-kDa heat shock protein and is a component of steroid receptor complexes.
PMID: 1376003 by Tai P.-K.K., et al. Association of a 59-kilodalton immunophilin with the glucocorticoid receptor complex.