GAK Antibodies

AND GAK ELISA kits, GAK Proteins

Many biological assays use antibodies to detect GAK, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with GAK in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop GAK antibodies...

We validate the specificity of these antibodies to GAK by testing them on tissues known to express GAK positively and negatively. Browse below to find the GAK antibody that suites your experiment. We have 4 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing an IHC experiment for GAK? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.

Open the GAK IHC Guide

GAK Infographic by Boster Bio

All GAK Products

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Product

Figures

Specificity

Applications

Price

Anti-GAK (Q129) Antibody

Immunohistochemistry (IHC) analyzes of GAK (Q129) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50

Human, Mouse

IHC

$405
Cat # A04246-1
100ul

GAK (NM_005255) Human Over-expression Lysate

Human

$960
Cat # LS002580
20 µg, 100 µg

Anti-Kinesin-like protein KIF13B KIF13B Antibody

Human

ELISA, IF, IHC, WB

$380
Cat # A06122-1
100ul

Anti-Kinesin-like protein KIF13B KIF13B Antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using KIF13B Antibody

Human, Mouse, Rat

ELISA, IF, IHC, ICC, WB

$335
Cat # A06122
100ul

GAK Overview

Facts about Cyclin-G-associated kinase.

GAK 3D structure. Source: alphafold

Cyclin-G-associated kinase (GAK)

Associates with cyclin G and CDK5.
Appears to act as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by Hsc70 in non-neuronal cells.

Expression oscillates slightly during the cell cycle, peaking at G1. .

Gene Information

Human
Gene Name:	GAK
Uniprot:	O14976
Entrez:	2580

Family

Belongs to:

protein kinase superfamily

Alternative Names

Auxillin 2; cyclin G associated kinase; cyclin-G-associated kinase; EC 2.7.11.1; FLJ16629; FLJ40395; GAK; MGC99654

Molecular Weight

Mass (kDA):
143.191 kDA

Genomic Context


Human

Location:	4p16.3
Sequence:	4; NC_000004.12 (849276..932330, complement)

Tissue Specificity

Ubiquitous. Highest in testis.

Subcellular Localizations

Cytoplasm, perinuclear region. Golgi apparatus, trans-Golgi network. Cell junction, focal adhesion. Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions.

Diseases

Parkinson Disease
Neoplasms
Malignant Neoplasms
Malignant Paraganglionic Neoplasm
Melanoma
Hepatitis
Hepatitis C
Malignant Neoplasm Of Prostate
Virus Diseases
Alzheimer's Disease
Cholangiocarcinoma
Malignant Bone Neoplasm

Drug-induced Liver Injury
Abscess
Down Syndrome
Malignant Tumor Of Cervix
Sarcoma
Carcinoma
Fibrosis

Interacting Proteins

ATP13A2
HSPA8
LRRK2

Pathways

Endocytosis
Clathrin-mediated Endocytosis
Pathogenesis
Receptor-mediated Endocytosis
Mitosis
Localization
Cell Proliferation
Cell Cycle
Rna Interference
Transport
Hyperphosphorylation
Secretory Pathway
G1 Phase

Aging
Neurogenesis
Metaphase
Membrane Budding
Chromosome Segregation
Cell Cycle Arrest
Fermentation

PTMs

Phosphorylation

Dephosphorylation

Cleavage

Research Areas

Cell Cycle and Replication

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/GAK

References

PMID: 9299234 by Kimura S.H., et al. Structure, expression, and chromosomal localization of human GAK.

PMID: 10625686 by Greener T., et al. Role of cyclin G-associated kinase in uncoating clathrin-coated vesicles from non-neuronal cells.