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- Table of Contents
HDAC6 · Western blot design guide
HDAC6 Western Blot Planning Guide
Plan a HDAC6 Western blot around the catalog-observed 131.4 kDa band, image-backed PB9628 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
HDAC6 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 131.4 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Testis | |
| Negative control | Bronchus |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 131.4 kDa |
| ⓘ | Localization | Cytoplasm / Cytoplasm, cytoskeleton |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated HDAC6 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Breast |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PB9628 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected HDAC6 Western Blot Band Size?
Use the product-observed 131.4 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 131.4 kDa | Matches the authoritative product WB observation. |
| 131.4 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected HDAC6 appearancePlan around 131.4 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 131.4 kDa; use this as the primary experimental expectation. |
| Calculated mass | 131.4 kDa from UniProt Q9UBN7; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PB9628. |
| Situation | Likely cause | Next action |
|---|---|---|
| 131.4 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing HDAC6 band
PB9628 · HDAC6
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for HDAC6 Western blot
🧪Use Testis as the first positive-control candidate and Bronchus as the HPA Not detected negative candidate.
Positive control: Testis (High)
Negative control: Bronchus (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for HDAC6
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Testis | Reported tissue cells | High | Protein (HPA) | HPA → |
| Breast | Reported tissue cells | High | Protein (HPA) | HPA → |
| Skin | Reported tissue cells | High | Protein (HPA) | HPA → |
| Adrenal gland | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 3
Advanced HDAC6 Western Blot Tips
Deeper troubleshooting and optimisation questions for HDAC6, answered from its protein features.
Which band should guide the blot?
Use 131.4 kDa, the observation attached to the authoritative PB9628 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 131.4 kDa expectation.
Which positive control should I start with?
Start with Testis, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Bronchus as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for HDAC6 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PB9628 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
HDAC6 Western Blot Reagents
Human/Mouse/Rat-reactive HDAC6 Western blot reagents with authoritative product imagery.
Real WB data
Anti-HDAC6 Antibody Picoband®
Cat # PB9628
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q9UBN7
- Human Protein Atlas — HDAC6
- PB9628 product record
- PMC11321199 — SARS-CoV-2 nucleocapsid protein promotes self-deacetylation by inducing HDAC6 to facilitate viral replication (Virology journal, 2024)
- PMC13541193 — Hyperglycemic stress aggravates diabetic retinopathy and nephropathy by promoting cilium disassembly via a deacetylation- and methylation-mediated regulatory mechanism (PLoS biology, 2026)
- PMC5541905 — ARID1A-mutated ovarian cancers depend on HDAC6 activity (Nature cell biology, 2017)
