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- Table of Contents
HIP1R · Western blot design guide
HIP1R Western Blot Planning Guide
Plan a HIP1R Western blot around the catalog-observed 119.4 kDa band, image-backed A05059-2 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
HIP1R Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 119.4 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Cerebellum | |
| Negative control | Skeletal muscle |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 119.4 kDa |
| ⓘ | Localization | Cytoplasm, perinuclear region / Endomembrane system |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated HIP1R Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Appendix |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A05059-2 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected HIP1R Western Blot Band Size?
Use the product-observed 119.4 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 119.4 kDa | Matches the authoritative product WB observation. |
| 119.4 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected HIP1R appearancePlan around 119.4 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 119.4 kDa; use this as the primary experimental expectation. |
| Calculated mass | 119.4 kDa from UniProt O75146; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A05059-2. |
| Situation | Likely cause | Next action |
|---|---|---|
| 119.4 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing HIP1R band
A05059-2 · HIP1R
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for HIP1R Western blot
🧪Use Cerebellum as the first positive-control candidate and Skeletal muscle as the HPA Not detected negative candidate.
Positive control: Cerebellum (High)
Negative control: Skeletal muscle (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for HIP1R
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Cerebellum | Reported tissue cells | High | Protein (HPA) | HPA → |
| Colon | Reported tissue cells | High | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | High | Protein (HPA) | HPA → |
| Bronchus | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 3
Advanced HIP1R Western Blot Tips
Deeper troubleshooting and optimisation questions for HIP1R, answered from its protein features.
Which band should guide the blot?
Use 119.4 kDa, the observation attached to the authoritative A05059-2 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 119.4 kDa expectation.
Which positive control should I start with?
Start with Cerebellum, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Skeletal muscle as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for HIP1R Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A05059-2 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
HIP1R Western Blot Reagents
Human/Mouse/Rat-reactive HIP1R Western blot reagents with authoritative product imagery.
Real WB data
Anti-HIP1R Antibody Picoband®
Cat # A05059-2
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt O75146
- Human Protein Atlas — HIP1R
- A05059-2 product record
- PMC10002968 — DNA methylation and miR-92a-3p-mediated repression of HIP1R promotes pancreatic cancer progression by activating the PI3K/AKT pathway (Journal of cellular and molecular medicine, 2023)
- PMC13194821 — Ceramide disrupts TM9SF2-PGK1 axis to redirect PD-L1 trafficking and enhance antitumor immunity (Nature communications, 2026)
- PMC12206818 — Therapeutic implications of cancer-associated fibroblast heterogeneity: insights from single-cell and multi-omics analysis (Frontiers in immunology, 2025)
