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- Table of Contents
HIP1R antibody (A05059-2) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Monkey, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of Raji cells using anti-HIP1R antibody (A05059-2).
Overlay histogram showing Raji cells stained with A05059-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HIP1R Antibody (A05059-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Raji cells stained with A05059-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HIP1R Antibody (A05059-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-HIP1R Antibody Picoband®
HIP1R antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
HIP1R antibody was positively detected by WB in:
human Hela whole cell, human PC-3 whole cell, monkey COS-7 whole cell, human T-47D whole cell, human Raji whole cell, rat brain tissue, rat heart tissue, rat pancreas tissue ltysates, mouse pancreas tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
HIP1R antibody was positively detected by IHC in:
human liver cancer tissue, human lung cancer tissue, human rectal cancer tissue, human renal clear cell carcinoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
HIP1R antibody was positively detected by ICC/IF in:
MCF-7 cell
HIP1R antibody was positively detected by FC in:
Raji cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat, Monkey
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Monkey
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Product Information
Product Name
Anti-HIP1R Antibody Picoband®
View all HIP1R Antibodies
View all HIP1R Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HIP1R recombinant protein (Position: E316-E734).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
120 kDa
Calculated Molecular Weight
119.4 kDa
Background of HIP1R
Huntingtin-interacting protein 1-related protein is a protein that in humans is encoded by the HIP1R gene. Huntingtin-interacting protein 1-related protein, or HIP1R, was identified on the basis of its structural homology with Huntingtin-interacting protein 1, or HIP1. Based on its domain structure, HIP1R is a putative endocytosis-related protein. Knockdown of HIP1R impairs the endocytosis of activated epidermal growth factor receptor (EGFR) and the consequent activation of the downstream ERK and Akt proteins. Additionally, HIP1R is a component of the clathrin-coated pits and vesicles, which in part links the endocytic machinery to the actin cytoskeleton. It binds to 3-phosphoinositides via ENTH domains to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis. HIP1R deficiency significantly reduces the expression of the ionotropic glutamate receptor GluA1, GluN2A, and GluN2B subunits, but not the GABAA receptor α1 subunit. Knockdown of HIP1R reduces the amplitude and frequency of the miniature excitatory postsynaptic current, but not of the miniature inhibitory postsynaptic curren. HIP1R has been identified as a protein that can target histone deacetylase-3-mediated neurodegeneration, along with other proteins like NPTX1, NFL, TEX10, and TGFFG.
Cite This Product
Anti-HIP1R Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05059-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
96%
High Homology
Rat
88%
Moderate Homology
Sheep
88%
Moderate Homology
Cow
87%
Moderate Homology
Mouse
87%
Moderate Homology
Dog
83%
Moderate Homology
Zebrafish
53%
Low homology
Drosophila
26%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for HIP1R antibody
Immunohistochemistry (IHC) protocol for HIP1R antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for HIP1R antibody
Flow Cytometry (FC) protocol for HIP1R antibody
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Product Promise
We promise Anti-HIP1R Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A05059-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026