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- Table of Contents
HK2 · Western blot design guide
HK2 Western Blot Planning Guide
Plan a HK2 Western blot around the catalog-observed 102.4 kDa band, image-backed A01389 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
HK2 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 102.4 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Epididymis | |
| Negative control | Oral mucosa |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 102.4 kDa |
| ⓘ | Localization | Mitochondrion outer membrane / Cytoplasm, cytosol |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated HK2 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Epididymis |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A01389 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected HK2 Western Blot Band Size?
Use the product-observed 102.4 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 102.4 kDa | Matches the authoritative product WB observation. |
| 102.4 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected HK2 appearancePlan around 102.4 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 102.4 kDa; use this as the primary experimental expectation. |
| Calculated mass | 102.4 kDa from UniProt P52789; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A01389. |
| Situation | Likely cause | Next action |
|---|---|---|
| 102.4 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing HK2 band
A01389 · HK2
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for HK2 Western blot
🧪Use Epididymis as the first positive-control candidate and Oral mucosa as the HPA Not detected negative candidate.
Positive control: Epididymis (High)
Negative control: Oral mucosa (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for HK2
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Epididymis | Reported tissue cells | High | Protein (HPA) | HPA → |
| Adrenal gland | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Caudate | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Cerebellum | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 3
Advanced HK2 Western Blot Tips
Deeper troubleshooting and optimisation questions for HK2, answered from its protein features.
Which band should guide the blot?
Use 102.4 kDa, the observation attached to the authoritative A01389 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 102.4 kDa expectation.
Which positive control should I start with?
Start with Epididymis, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Oral mucosa as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for HK2 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A01389 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
HK2 Western Blot Reagents
Human/Mouse/Rat-reactive HK2 Western blot reagents with authoritative product imagery.
Real WB data
Anti-Hexokinase II/HK2 Antibody Picoband®
Cat # A01389
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P52789
- Human Protein Atlas — HK2
- A01389 product record
- PMC3753022 — Hexokinase 2 is required for tumor initiation and maintenance and its systemic deletion is therapeutic in mouse models of cancer (Cancer cell, 2013)
- PMC11955892 — [High expression of hexokinase 2 promotes proliferation, migration and invasion of colorectal cancer cells by activating the JAK/STAT pathway and regulating tumor immune microenvironment] (Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2025)
- PMC9334759 — IL13Rα1 prevents a castration resistant phenotype of prostate cancer by targeting hexokinase 2 for ubiquitin-mediated degradation (Cancer biology & medicine, 2021)