HTR4 Antibodies

AND HTR4 ELISA kits, HTR4 Proteins

Many biological assays use antibodies to detect HTR4, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with HTR4 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop HTR4 antibodies...

We validate the specificity of these antibodies to HTR4 by testing them on tissues known to express HTR4 positively and negatively. Browse below to find the HTR4 antibody that suites your experiment. We have 3 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing an IHC experiment for HTR4? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.

Open the HTR4 IHC Guide

HTR4 Infographic by Boster Bio

All HTR4 Products

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Product

Figures

Specificity

Applications

Price

Anti-SR-4 HTR4 Antibody

Western Blot analysis of various cells using SR-4 Polyclonal Antibody

Western blot analysis of lysates from Jurkat/COLO205, using 5-HT-4 Antibody

Western blot analysis of the lysates from 293 cells using Collagen I α2 antibody

Human, Mouse, Rat

ELISA, IF, IHC, WB

$399
Cat # A03681-1
100μl

Anti-SR-4 (D47) HTR4 Antibody

Western blot (WB) analysis of SR-4 (D47) pAb at 1:500 dilution
Lane1:CT26 whole cell lysate(40ug)
Lane2:C6 whole cell lysate(40ug)
Lane3:HEK293T whole cell lysate(40ug)
Lane4:K562 whole cell lysate(40ug)
Lane5:U-87MG whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of SR-4 (D47) pAb in paraffin-embedded human brain tissue

Human, Mouse, Rat

IF, IHC, WB

$405
Cat # A03681-3
100ul

5HT4 Receptor (HTR4) (NM_000870) Human Over-expression Lysate

5HT4 Receptor (HTR4) Human Over-expression Lysates NM_000870

Human

$628
Cat # LS003360
20 µg, 100 µg

HTR4 Overview

Facts about 5-hydroxytryptamine receptor 4.

HTR4 3D structure. Source: alphafold

5-hydroxytryptamine receptor 4 (HTR4)

This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase.

Gene Information

Human
Gene Name:	HTR4
Uniprot:	Q13639
Entrez:	3360

Family

Belongs to:

G-protein coupled receptor 1 family

Alternative Names

5HT4; 5-HT4; 5-HT-4; 5-HT4R; 5-hydroxytryptamine (serotonin) receptor 4,5-hydroxytryptamine receptor 4,5-HT45-hydroxytryptamine4 receptor; cardiac 5-HT4 receptor; HTR4; Serotonin receptor 4

Molecular Weight

Mass (kDA):
43.761 kDA

Genomic Context


Human

Location:	5q32
Sequence:	5; NC_000005.10 (148451032..148654527, complement)

Tissue Specificity

Isoform 5-HT4(A) is expressed in ileum, brain, and atrium, but not in the ventricle.

Subcellular Localizations

Cell membrane; Multi-pass membrane protein. Endosome. Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli.

Diseases

Schizophrenia
Lung Diseases
Attention Deficit Hyperactivity Disorder
Lung Diseases, Obstructive
Chronic Obstructive Airway Disease
Nervousness
Bipolar Disorder
Dysequilibrium Syndrome
Chronic Fatigue Syndrome
Malnutrition
Mood Disorders
Communicable Diseases
Chronic Lung Disease

Respiration Disorders
Hypokinesia
Endoplasmic Reticulum Stress
Cardiovascular Diseases
Gastrointestinal Symptom
Asthma
Chromosome Breakage

Interacting Proteins

GPR37
GPRIN2

Pathways

Localization
Methylation
Transport
Gastric Emptying
Response To Stress
Cellular Localization
Brain Development
Immune Response
Glycosylation

Cognition
Pathogenesis
Rna Splicing

PTMs

Methylation

Research Areas

GPCR
Neuronal Cell Markers
Neuroscience
Neurotransmission

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/HTR4

References

PMID: 9603189 by Blondel O., et al. Cloning, expression, and pharmacology of four human 5- hydroxytryptamine 4 receptor isoforms produced by alternative splicing in the carboxyl terminus.

PMID: 9351641 by Claeysen S., et al. Cloning and expression of human 5-HT4S receptors. Effect of receptor density on their coupling to adenylyl cyclase.