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Product Info Summary
| SKU: | A03681-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-SR-4 HTR4 Antibody
SKU/Catalog Number
A03681-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-SR-4 HTR4 Antibody catalog # A03681-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SR-4 HTR4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03681-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human 5-HT-4. AA range:21-70
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03681-1 is reactive to HTR4 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
43.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03681-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using SR-4 Polyclonal Antibody
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Western blot analysis of lysates from Jurkat/COLO205, using 5-HT-4 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from 293 cells using Collagen I α2 antibody.
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Immunofluorescence analysis of HeLa cells, using 5-HT-4 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-SR-4 HTR4 Antibody (A03681-1)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-SR-4 HTR4 Antibody
Question
Does anti-SR-4 antibody A03681-1 work on pig WB with brain?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
Our lab technicians have not tested anti-SR-4 antibody A03681-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-SR-4 antibody A03681-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-09
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for epithelium of bronchus using anti-SR-4 antibody A03681-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-30
Question
Would A03681-1 anti-SR-4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Wu
Verified customer
Asked: 2018-12-06
Answer
As indicated on the product datasheet, A03681-1 anti-SR-4 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-12-06
Question
Is this A03681-1 anti-SR-4 antibody reactive to the isotypes of HTR4?
Verified Customer
Verified customer
Asked: 2018-05-11
Answer
The immunogen of A03681-1 anti-SR-4 antibody is Synthesized peptide derived from the N-terminal region of human SR-4. at AA rangle: 40-120. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-05-11
Question
I am looking for to test anti-SR-4 antibody A03681-1 on mouse epithelium of bronchus for research purposes, then I may be interested in using anti-SR-4 antibody A03681-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
W. Johnson
Verified customer
Asked: 2017-09-01
Answer
The products we sell, including anti-SR-4 antibody A03681-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-09-01
Question
Can you help my question with product A03681-1, anti-SR-4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Walker
Verified customer
Asked: 2016-02-09
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03681-1 anti-SR-4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-02-09
Question
Is a blocking peptide available for product anti-SR-4 antibody (A03681-1)?
V. Johnson
Verified customer
Asked: 2013-04-22
Answer
We do provide the blocking peptide for product anti-SR-4 antibody (A03681-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2013-04-22
