IER3 Antibodies

AND IER3 ELISA kits, IER3 Proteins

Many biological assays use antibodies to detect IER3, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with IER3 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop IER3 antibodies...

We validate the specificity of these antibodies to IER3 by testing them on tissues known to express IER3 positively and negatively. Browse below to find the IER3 antibody that suites your experiment. We have 4 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing an IHC experiment for IER3? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.

Open the IER3 IHC Guide

IER3 Infographic by Boster Bio

All IER3 Products

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Product

Figures

Specificity

Applications

Price

Anti-IER3 Antibody Picoband®

Western blot analysis of IER3 using anti-IER3 antibody (A04014-1)

IHC analysis of IER3 using anti-IER3 antibody (A04014-1)

Flow Cytometry analysis of Jurkat cells using anti-IER3 antibody (A04014-1)

Human

Flow Cytometry, IHC, WB

$370
Cat # A04014-1
100 μg/vial

Anti-IEX-1 IER3 Antibody

Western blot analysis of IEX-1 in mouse skin tissue lysate with IEX-1 antibody at (A) 1 and (B) 2 μg/mL

Immunohistochemistry of IEX-1 in human liver tissue with IEX-1 antibody at 2

Immunofluorescence of IEX-1 in Human Brain cells with IEX-1 antibody at 20 μg/mL

Human, Mouse

ELISA, IF, IHC-P, WB

$481
Cat # A04014
0.1 mg

IEX1 (IER3) (NM_003897) Human Over-expression Lysate

Human

$628
Cat # LS008870
20 µg, 100 µg

Human IER3 Recombinant Protein

SDS-PAGE

$268
Cat # PROTP46695
Starting from 10ug

IER3 Overview

Facts about Radiation-inducible immediate-early gene IEX-1.

IER3 3D structure. Source: alphafold

Radiation-inducible immediate-early gene IEX-1 (IER3)

May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A- PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival.

As a member of the NUPR1/RELB/IER3 survival pathway, can offer pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine therapy. .

Gene Information

Human
Gene Name:	IER3
Uniprot:	P46695
Entrez:	8870

Family

Belongs to:

IER3 family

Alternative Names

DIF-2; DIF2GLY96; Differentiation-dependent gene 2 protein; expressed in pancreatic carcinoma; gly96, mouse, homolog of; IEX-1; IEX1death protein; IEX-1L; Immediate early protein GLY96; Immediate early response 3 protein; immediate early response 3; immediately early gene X-1; PRG1PACAP-responsive gene 1 protein; Protein DIF-2; Protein PRG1; radiation-inducible immediate-early gene IEX-1

Molecular Weight

Mass (kDA):
16.903 kDA

Genomic Context


Human

Location:	6p21.33
Sequence:	6; NC_000006.12 (30743199..30744547, complement)

Subcellular Localizations

Membrane; Single-pass type II membrane protein.

Diseases

Neoplasms
Malignant Neoplasms
Carcinoma
Cell Transformation, Neoplastic
Adenocarcinoma
Malignant Neoplasm Of Breast
Malignant Paraganglionic Neoplasm
Mammary Neoplasms
Carcinogenesis
Virus Diseases
Leukemia
Malignant Neoplasm Of Pancreas

Inflammation
Pancreatic Neoplasm
Dysmyelopoietic Syndromes
Hypoxia
Acute Promyelocytic Leukemia
Pituitary Diseases
Hypertrophy

Interacting Proteins

CAMLG
PPP2CA
PPP2R5B
PPP2R5C
RELA

SGTA

Pathways

Cell Cycle
Cell Growth
Cell Proliferation
Cell Death
Localization
Cell Differentiation
Cell Cycle Arrest
Viral Replication
Oncogenesis
Pathogenesis
Immune Response
Regulation Of Gene Expression
Chemotaxis

Translation
Induction Of Apoptosis
Regulation Of Cell Proliferation
Bone Remodeling
Angiogenesis
Rna Interference
Cell Adhesion

PTMs

Phosphorylation
Cleavage
Glycosylation

Amidation
Methylation
Dephosphorylation

Research Areas

Apoptosis

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/IER3

References

PMID: 8603392 by Kondratyev A.D., et al. Identification and characterization of a radiation-inducible glycosylated human early-response gene.

PMID: 8653710 by Schaefer H., et al. PRG1: a novel early-response gene transcriptionally induced by pituitary adenylate cyclase activating polypeptide in a pancreatic carcinoma cell line.