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- Table of Contents
INF2 · Western blot design guide
INF2 Western Blot Planning Guide
Plan a INF2 Western blot around the catalog-observed 135.6 kDa band, image-backed A02710-2 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
INF2 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 135.6 kDa observed | |
| Observed band | 135.6 kDa observed | |
| Gel | 8-10% | |
| Positive control | Gallbladder | |
| Negative control | Heart muscle |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 135.6 kDa |
| ⓘ | Localization | Cytoplasm, perinuclear region |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated INF2 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Appendix |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A02710-2 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected INF2 Western Blot Band Size?
Use the product-observed 135.6 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 135.6 kDa | Matches the authoritative product WB observation. |
| 135.6 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected INF2 appearancePlan around 135.6 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 135.6 kDa; use this as the primary experimental expectation. |
| Calculated mass | 135.6 kDa from UniProt Q27J81; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A02710-2. |
| Situation | Likely cause | Next action |
|---|---|---|
| 135.6 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing INF2 band
A02710-2 · INF2
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for INF2 Western blot
🧪Use Gallbladder as the first positive-control candidate and retain an orthogonal negative control.
Positive control: Gallbladder (High)
Negative control: Heart muscle (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for INF2
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Gallbladder | Reported tissue cells | High | Protein (HPA) | HPA → |
| Stomach | Reported tissue cells | High | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | High | Protein (HPA) | HPA → |
| Placenta | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 3
Advanced INF2 Western Blot Tips
Deeper troubleshooting and optimisation questions for INF2, answered from its protein features.
Which band should guide the blot?
Use 135.6 kDa, the observation attached to the authoritative A02710-2 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 135.6 kDa expectation.
Which positive control should I start with?
Start with Gallbladder, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Heart muscle as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for INF2 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A02710-2 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
INF2 Western Blot Reagents
Human/Mouse/Rat-reactive INF2 Western blot reagents with authoritative product imagery.
Real WB data
Anti-INF2 Antibody Picoband®
Cat # A02710-2
Real WB data
Anti-INF2 Antibody Picoband®
Cat # A02710-1
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q27J81
- Human Protein Atlas — INF2
- A02710-2 product record
- PMC4884070 — An mDia1-INF2 formin activation cascade facilitated by IQGAP1 regulates stable microtubules in migrating cells (Molecular biology of the cell, 2016)
- PMC8144320 — Enterovirus 71 Induces INF2 Cleavage via Activated Caspase-2 in Infected RD Cells (Frontiers in microbiology, 2021)
- PMC5363079 — Mice with mutant Inf2 show impaired podocyte and slit diaphragm integrity in response to protamine-induced kidney injury (Kidney international, 2016)