Josephin-1 Antibodies

AND JOSD1 ELISA kits, Josephin-1 Proteins

Many biological assays use antibodies to detect Josephin-1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Josephin-1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Josephin-1 antibodies...

We validate the specificity of these antibodies to Josephin-1 by testing them on tissues known to express JOSD1 positively and negatively. Browse below to find the JOSD1 antibody that suites your experiment. We have 3 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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JOSD1 Infographic by Boster Bio

All JOSD1 Products

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Product

Figures

Specificity

Applications

Price

Human Josephin-1 Recombinant Protein

SDS-PAGE

$250
Cat # PROTQ15040
Starting from 20ug

Anti-Josephin 1 (JOSD1) Mouse Monoclonal Antibody [Clone ID: OTI3B11]

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JOSD1 (Right lane) cDNA for 48 hrs and lysed

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-JOSD1 mouse monoclonal antibody

Human, Rat

IHC, WB

$523
Cat # M12300
100 µl

Josephin 1 (JOSD1) (NM_014876) Human Over-expression Lysate

Human

$628
Cat # LS009929
20 µg, 100 µg

JOSD1 Overview

Facts about Josephin-1.

JOSD1 3D structure. Source: alphafold

Josephin-1 (JOSD1)

Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves'Lys-63'-linked and'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme.

May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity.

Gene Information

Human
Gene Name:	JOSD1
Uniprot:	Q15040
Entrez:	9929

Family

Belongs to:

No superfamily

Alternative Names

EC 3.4.19.12; JOSD1; Josephin domain containing 1; Josephin domain-containing 1; Josephin-1; JSPH1; KIAA0063dJ508I15.2

Molecular Weight

Mass (kDA):
23.198 kDA

Genomic Context


Human

Location:	22q13.1
Sequence:	22; NC_000022.11 (38683916..38701403, complement)

Subcellular Localizations

Cell membrane. Cytoplasm. Ubiquitination increases localization the plasma membrane. In the cytosol, the unubiquitinated form may be associated with the cytoskeleton via ACTB-binding.

Diseases

Leukemia, Myelocytic, Acute

Interacting Proteins

KRT31
KRT40
KRTAP10-4
KRTAP10-8
KRTAP5-9

KRTAP9-2
NOTCH2NL
TRAF1
TRIM23
TRIM27

Pathways

Bioluminescence
Conjugation

PTMs

Phosphoprotein

Ubl conjugation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/JOSD1

References

PMID: 17696782 by Tzvetkov N., et al. Josephin domain-containing proteins from a variety of species are active de-ubiquitination enzymes.

PMID: 21118805 by Weeks S.D., et al. Crystal structure of a Josephin-ubiquitin complex: evolutionary restraints on ataxin-3 deubiquitinating activity.