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- Table of Contents
KCNA4 · Western blot design guide
KCNA4 Western Blot Planning Guide
Plan a KCNA4 Western blot around the catalog-observed 73.3 kDa band, image-backed PA2297 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
KCNA4 Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 73.3 kDa observed | |
| Observed band | 73.3 kDa observed | |
| Gel | 12-15% | |
| Positive control | Adrenal gland | |
| Negative control | Appendix |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 73.3 kDa |
| ⓘ | Localization | Cell membrane / Cell projection, axon |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated KCNA4 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Adrenal gland |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PA2297 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected KCNA4 Western Blot Band Size?
Use the product-observed 73.3 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 73.3 kDa | Matches the authoritative product WB observation. |
| 73.3 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected KCNA4 appearancePlan around 73.3 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 73.3 kDa; use this as the primary experimental expectation. |
| Calculated mass | 73.3 kDa from UniProt P22459; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PA2297. |
| Situation | Likely cause | Next action |
|---|---|---|
| 73.3 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing KCNA4 band
PA2297 · KCNA4
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for KCNA4 Western blot
🧪Use Adrenal gland as the first positive-control candidate and retain an orthogonal negative control.
Positive control: Adrenal gland (Medium)
Negative control: Appendix (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for KCNA4
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adrenal gland | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Hippocampus | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Cerebellum | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Cerebral cortex | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 3
Advanced KCNA4 Western Blot Tips
Deeper troubleshooting and optimisation questions for KCNA4, answered from its protein features.
Which band should guide the blot?
Use 73.3 kDa, the observation attached to the authoritative PA2297 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 73.3 kDa expectation.
Which positive control should I start with?
Start with Adrenal gland, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Appendix as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for KCNA4 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PA2297 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
KCNA4 Western Blot Reagents
Human/Mouse/Rat-reactive KCNA4 Western blot reagents with authoritative product imagery.
Real WB data
Anti-Kv1.4/KCNA4 Antibody Picoband®
Cat # PA2297
Real WB data
Anti-Kv1.4/KCNA4 Antibody Picoband®
Cat # PB9807
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P22459
- Human Protein Atlas — KCNA4
- PA2297 product record
- PMC10149585 — miR-448 regulates potassium voltage-gated channel subfamily A member 4 (KCNA4) in ischemia and heart failure (Heart rhythm, 2023)