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- Table of Contents
KDM5B · Western blot design guide
KDM5B Western Blot Planning Guide
Plan a KDM5B Western blot around the catalog-observed 175.7 kDa band, image-backed PB10073 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
KDM5B Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 175.7 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Testis | |
| Negative control | Cerebral cortex |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 175.7 kDa |
| ⓘ | Localization | Nucleus |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Sample controls for KDM5B Western blot
🧪Use Testis as the first positive-control candidate and Cerebral cortex as the HPA Not detected negative candidate.
Positive control: Testis (High)
Negative control: Cerebral cortex (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for KDM5B
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Testis | Reported tissue cells | High | Protein (HPA) | HPA → |
| Fallopian tube | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Lung | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Bronchus | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 1
What Is the Expected KDM5B Western Blot Band Size?
Use the product-observed 175.7 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 175.7 kDa | Matches the authoritative product WB observation. |
| 175.7 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected KDM5B appearancePlan around 175.7 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 175.7 kDa; use this as the primary experimental expectation. |
| Calculated mass | 175.7 kDa from UniProt Q9UGL1; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PB10073. |
| Situation | Likely cause | Next action |
|---|---|---|
| 175.7 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing KDM5B band
PB10073 · KDM5BObserved 175.7 kDa · product-specific WB image
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Section 2
Real Curated KDM5B Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Testis |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PB10073 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 4
Advanced KDM5B Western Blot Tips
Deeper troubleshooting and optimisation questions for KDM5B, answered from its protein features.
Which band should guide the blot?
Use 175.7 kDa, the observation attached to the authoritative PB10073 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 175.7 kDa expectation.
Which positive control should I start with?
Start with Testis, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Cerebral cortex as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for KDM5B Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PB10073 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
Compare only the supplied target-verified records: PMC11436259, PMC6036731, PMC10492338.
Boster reagents
KDM5B Western Blot Reagents
Human/Mouse/Rat-reactive KDM5B Western blot reagents with authoritative product imagery.
Real WB data
Anti-KDM5B/PLU1/Jarid1B Antibody Picoband®
Cat # PB10073
Reactivity: Human, Monkey, Mouse, Rat
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q9UGL1
- Human Protein Atlas — KDM5B
- PB10073 product record
- PMC11436259 — AUP1 transcriptionally activated by KDM5B reprograms lipid metabolism to promote the malignant progression of cervical cancer.
- PMC6036731 — KDM5B demethylates H3K4 to recruit XRCC1 and promote chemoresistance.
- PMC10492338 — Echinococcus granulosus cyst fluid inhibits inflammatory responses through inducing histone demethylase KDM5B in macrophages.