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- Table of Contents
LPCAT1 / Lysophosphatidylcholine acyltransferase 1 · Western blot design guide
Design a Western Blot for LPCAT1
Source-linked LPCAT1 Western blot protocol options, expected-band and isoform facts, troubleshooting for weak or shifted signal, and recommended anti-LPCAT1 WB antibodies. Everything you need to plan the experiment before you commit precious samples.
Evidence assembled September 2026 · For research use; verify linked source records and product datasheet before use
LPCAT1 Western Blot Experimental Design Guide
Expected bands, source-linked protocol options, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | ~59.2 kDa | |
| Observed band | ~59 kDa | |
| Gel | 10% (catalog A04863-1) | |
| Positive control | Bronchus (IHC candidate; verify WB) +4 more | |
| Negative control | Adipose tissue (IHC candidate; verify WB) |
Important caveats
Reasons your observed band may differ from the expected size.
| PTM | — | |
| Caveat | — | |
| Gene-set association | MSigDB C7 membership | |
| Isoform | 1 isoform(s) |
Section 1
Source-Linked LPCAT1 Western Blot Protocol Options
The A04863-1 protocol combines labelled catalog values with standard starting conditions. Published comparisons retain their own sample, reagent and detection scope.
| Sample / lysate | human A431, human A549, human PC-3, human Hela, rat lung, mouse lung (catalog A04863-1) |
| Gel % | 10% (catalog A04863-1) |
| Load | 30 ug; reducing conditions (catalog A04863-1) |
| Transfer | a nitrocellulose membrane at 150 mA for 50-90 minutes (catalog A04863-1) |
| Membrane | nitrocellulose membrane (catalog A04863-1) |
| Blocking | 5% non-fat milk/TBS for 1.5 hour at RT (catalog A04863-1) |
| Primary antibody | A04863-1 · 1: 1000 (catalog A04863-1) |
| Primary incubation | overnight at 4°C (catalog A04863-1) |
| Secondary antibody | goat anti-rabbit IgG-HRP, 1:5000 (catalog A04863-1) |
| Secondary incubation | 1.5 hour at RT (catalog A04863-1) |
| Wash | TBS-0.1%Tween 3 times with 5 minutes each (catalog A04863-1) |
| Detection | ECL (catalog A04863-1) |
Section 2
What Is the Expected LPCAT1 Western Blot Band Size?
LPCAT1 is predicted at 59.2 kDa and observed at approximately 59 kDa; the small difference has no established cause in the supplied evidence.
| Band at approximately 59 kDa | Matches the empirical LPCAT1 band and its 59.2 kDa predicted mass |
| Single sharp band near 59 kDa | Consistent with the predicted size and absence of listed size-modifying features |
| Weak band near 59 kDa in whole-cell lysate | Membrane-associated LPCAT1 may be incompletely recovered |
| Band near 59 kDa in a membrane fraction | Consistent with LPCAT1 membrane localization |
💡Expected LPCAT1 appearanceLPCAT1 has a predicted mass of 59.2 kDa and an empirical band at approximately 59 kDa; confirm identity with appropriate antibody and sample controls.
| UniProt predicted molecular weight | Places full-length LPCAT1 near 59.2 kDa |
| UniProt mass of 59,151 Da | Corresponds to approximately 59.2 kDa |
| Predicted mass versus empirical band | The 59.2 kDa prediction closely matches the approximately 59 kDa observed band |
| Predicted mass as band reference | Bands far from 59.2 kDa require independent identity checks |
| Situation | Likely cause | Next action |
|---|---|---|
| No band in lysate | Membrane-associated LPCAT1 may be incompletely extracted | Check membrane protein recovery and test a membrane-enriched fraction |
| Weak or no signal | Limited recovery of membrane-associated LPCAT1 | Check extraction and loading with a membrane protein control |
| Band higher than expected | The supplied features do not establish its identity | Compare with the approximately 59 kDa band using an independent antibody or depletion control |
| Band lower than expected | The supplied features do not establish its identity | Compare with the approximately 59 kDa band using an independent antibody or depletion control |
| Multiple bands | Additional bands are unexplained by the listed features | Verify the approximately 59 kDa band with an independent antibody or depletion control |
Experiment evidence
Western blot images showing LPCAT1 band
A04863-1 · LPCAT1
A04863 · LPCAT1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for LPCAT1 Western blot
🧪HPA-IHC candidate guidance (verify in WB): For positive controls for LPCAT1 in Western blot, you can use bronchus tissue, which shows high HPA expression.
Positive control: Bronchus (IHC candidate; verify WB)
Negative control: Adipose tissue (IHC candidate; verify WB)
Loading controls: Run GAPDH, β-actin, and a total-protein stain alongside the samples.
⚠️Feasibility: LPCAT1 is membrane-associated, so use lysates that retain membrane proteins.
HPA tissue expression evidence for LPCAT1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Higher expression tissues · candidate positive controls from IHC
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Bronchus | basal cells | High | Protein (IHC) | HPA → |
| Lung | alveolar cells type I | High | Protein (IHC) | HPA → |
| Nasopharynx | basal cells | High | Protein (IHC) | HPA → |
| Skin | fibrohistiocytic cells | High | Protein (IHC) | HPA → |
| Testis | Leydig cells | High | Protein (IHC) | HPA → |
Lower expression tissues · IHC evidence, not confirmed WB-negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adipose tissue | adipocytes | Not detected | Protein (IHC) | HPA → |
| Oral mucosa | squamous epithelial cells | Not detected | Protein (IHC) | HPA → |
| Vagina | squamous epithelial cells | Not detected | Protein (IHC) | HPA → |
| Bone marrow | hematopoietic cells | Low | Protein (IHC) | HPA → |
| Caudate | glial cells | Low | Protein (IHC) | HPA → |
Section 3
Advanced LPCAT1 Western Blot Tips
Deeper troubleshooting and optimisation questions for LPCAT1, answered from its protein features.
How should LPCAT1 band migration be interpreted?
Band shift · Use the separately labelled calculated mass and catalog-observed evidence above. A sequence annotation does not establish an observed migration shift. Verify target identity with orthogonal controls.
Could annotated isoforms explain multiple LPCAT1 bands?
Isoforms · Only one isoform is supplied, with no alternative sequence. The supplied features therefore do not identify an isoform that explains an additional band.
Do annotated modifications explain a shifted LPCAT1 band?
PTM · No modified residues or glycosylation sites are supplied. The supplied features do not support assigning a shifted band to a specific modification.
Does this guide establish induction of LPCAT1?
Induction · No general induction response is established by this guide. A pathway or gene-set association is not evidence of induction in a particular specimen. Verify the relevant treatment and control in a target-specific experiment.
What transfer method to use for LPCAT1 Western blot?
Transfer · LPCAT1 is annotated as a 59.2 kDa single-pass membrane protein. These features do not establish one best transfer method. Check transfer efficiency near 59 kDa when setting up the blot.
How should blocking be optimized?
Blocking · Standard workflow guidance: follow the A04863-1 datasheet where specified. Otherwise compare 5% milk or 5% BSA in TBST; for a phospho-specific assay start with BSA. Optimize background and specific signal with matched controls.
How should LPCAT1 bands be quantified?
Quantitation · Quantify the band near the supplied observed position of 59 kDa, using the same band definition across samples. The features alone do not establish that other bands represent LPCAT1.
Should LPCAT1 migrate above its predicted mass?
Interpretation · The predicted mass is 59.2 kDa, and the supplied observed band is about 59 kDa. The features do not establish a reason to expect a shift.
Compare it with the approximately 59 kDa observed band. One isoform is supplied, and no modified residues or glycosylation sites are annotated, so the supplied features do not identify the cause of an additional band.
Compare it with the approximately 59 kDa observed band. One isoform is supplied, and no modified residues or glycosylation sites are annotated, so the supplied features do not identify the cause of an additional band.
Boster reagents
LPCAT1 Western Blot Antibodies
Catalog antibodies with Western blot application and product-specific WB images. Evaluate suitability with the reported sample, controls and experimental conditions.
Real WB data
Anti-LPCAT1 Antibody
Cat # A04863-1
Real WB data
Anti-LPCAT1 Antibody
Cat # A04863
Two the supplier anti-LPCAT1 antibodies, A04863-1 and A04863, list Human, Mouse, and Rat reactivity and include Western blot images. A04863-1 documents named human cell and rat and mouse lung lysates; A04863 describes only ‘various lysates,’ limiting sample-specific interpretation.
Which to pick: Both list the same species and have WB images. Choose A04863-1 when its documented A431, A549, PC-3, HeLa, rat lung, or mouse lung conditions match your experiment; its caption reports a band near 59 kDa. A04863 gives fewer sample details.
Source: BosterBio LPCAT1 gene-info card — filtered to Western-blot-capable antibodies; each card shows that product's actual WB validation figure.
References
- UniProt Consortium. UniProt entry Q8NF37.
- Human Protein Atlas. LPCAT1 tissue expression.
- PMC4372572 — target-verified WB comparison
- PMC12678303 — target-verified WB comparison
- PMC13169525 — target-verified WB comparison