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- Table of Contents
LRRK2 · Western blot design guide
LRRK2 Western Blot Planning Guide
Plan a LRRK2 Western blot around the catalog-observed 286.1 kDa band, image-backed PB9281 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
LRRK2 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 286.1 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 4-12% gradient | |
| Positive control | Kidney | |
| Negative control | Bone marrow |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 286.1 kDa |
| ⓘ | Localization | Cytoplasmic vesicle / Perikaryon |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated LRRK2 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Kidney |
| Gel % | 4-12% gradient |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PB9281 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected LRRK2 Western Blot Band Size?
Use the product-observed 286.1 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 286.1 kDa | Matches the authoritative product WB observation. |
| 286.1 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected LRRK2 appearancePlan around 286.1 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 286.1 kDa; use this as the primary experimental expectation. |
| Calculated mass | 286.1 kDa from UniProt Q5S007; retain as context. |
| Gel selection | 4-12% gradient; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PB9281. |
| Situation | Likely cause | Next action |
|---|---|---|
| 286.1 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing LRRK2 band
PB9281 · LRRK2
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for LRRK2 Western blot
🧪Use Kidney as the first positive-control candidate and Bone marrow as the HPA Not detected negative candidate.
Positive control: Kidney (High)
Negative control: Bone marrow (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for LRRK2
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Kidney | Reported tissue cells | High | Protein (HPA) | HPA → |
| Lung | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Caudate | Reported tissue cells | Low | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | Low | Protein (HPA) | HPA → |
Section 3
Advanced LRRK2 Western Blot Tips
Deeper troubleshooting and optimisation questions for LRRK2, answered from its protein features.
Which band should guide the blot?
Use 286.1 kDa, the observation attached to the authoritative PB9281 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 286.1 kDa expectation.
Which positive control should I start with?
Start with Kidney, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Bone marrow as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 4-12% gradient consistently across the quick facts, protocol table, and poster.
What transfer method to use for LRRK2 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PB9281 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
LRRK2 Western Blot Reagents
Human/Mouse/Rat-reactive LRRK2 Western blot reagents with authoritative product imagery.
Real WB data
Anti-LRRK2 Antibody Picoband®
Cat # PB9281
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q5S007
- Human Protein Atlas — LRRK2
- PB9281 product record
- PMC7702304 — Endogenous Rab29 does not impact basal or stimulated LRRK2 pathway activity (The Biochemical journal, 2020)
- PMC10907374 — G2019S selective LRRK2 kinase inhibitor abrogates mitochondrial DNA damage (NPJ Parkinson's disease, 2024)
- PMC3429506 — Screening for novel LRRK2 inhibitors using a high-throughput TR-FRET cellular assay for LRRK2 Ser935 phosphorylation (PloS one, 2012)