MAP4K4 Antibodies

AND MAP4K4 ELISA kits, MAP4K4 Proteins

Many biological assays use antibodies to detect MAP4K4, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with MAP4K4 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop MAP4K4 antibodies...

We validate the specificity of these antibodies to MAP4K4 by testing them on tissues known to express MAP4K4 positively and negatively. Browse below to find the MAP4K4 antibody that suites your experiment. We have 2 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing an IHC experiment for MAP4K4? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.

Open the MAP4K4 IHC Guide

MAP4K4 Infographic by Boster Bio

All MAP4K4 Products

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Product

Figures

Specificity

Applications

Price

Anti-MAP4K4 Antibody Picoband®

Western blot analysis of MAP4K4 using anti-MAP4K4 antibody (A02829-2)

IHC analysis of MAP4K4 using anti-MAP4K4 antibody (A02829-2)

Human, Mouse, Rat

Flow Cytometry, IHC, WB

$370
Cat # A02829-2
100 μg/vial

MAP4K4 (NM_145686) Human Over-expression Lysate

Human

$960
Cat # LS009448
20 µg, 100 µg

MAP4K4 Overview

Facts about Mitogen-activated protein kinase kinase kinase kinase 4.

MAP4K4 3D structure. Source: alphafold

Mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4)

Serine/threonine kinase that may play a role in the response to environmental stress and cytokines like TNF-alpha. Appears to act upstream of the JUN N-terminal pathway.

Phosphorylates SMAD1 on Thr-322. .

Gene Information

Human
Gene Name:	MAP4K4
Uniprot:	O95819
Entrez:	9448

Family

Belongs to:

protein kinase superfamily

Alternative Names

EC 2.7.11; EC 2.7.11.1; FLH21957; FLJ10410; hepatocyte progenitor kinase-like/germinal center kinase-like kinase; HGK; HGKFLJ20373; HPK/GCK-like kinase HGK; KIAA0687; MAP4K4; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; MEKKK4; mitogen-activated protein kinase kinase kinase kinase 4; Nck-interacting kinase; NIKFLJ90111

Molecular Weight

Mass (kDA):
142.101 kDA

Genomic Context


Human

Location:	2q11.2
Sequence:	2; NC_000002.12 (101697703..101894690)

Tissue Specificity

Widely expressed. Isoform 5 is abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.

Subcellular Localizations

Cytoplasm.

Diseases

Malignant Neoplasms
Neoplasms
Inflammation
Diabetes Mellitus
Insulin Resistance
Carcinoma
Diabetes Mellitus, Non-insulin-dependent
Cell Invasion
Cell Transformation, Neoplastic
Tissue Adhesions
Obesity
Neoplasm Invasiveness
Adenocarcinoma

Neoplasm Metastasis
Malignant Neoplasm Of Breast
Epithelial Ovarian Cancer
Ovarian Neoplasm
Pancreatic Ductal Adenocarcinoma
Liver Carcinoma
Metaplastic Cell Transformation

Interacting Proteins

GBP3

Pathways

Rna Interference
Cell Migration
Cell Death
Transport
Gene Silencing
Cell Cycle
Cell Motility
Cell Differentiation
Cell Proliferation
Pathogenesis
Glycolysis
Glucose Transport
Insulin Secretion

Secretion
Cell Adhesion
Neural Tube Closure
Chemokine Production
Mesoderm Formation
Glucose Homeostasis
Exocytosis

PTMs

Phosphorylation

Methylation

Oxidation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/MAP4K4

References

PMID: 9890973 by Yao Z., et al. A novel human STE20-related protein kinase, HGK, that specifically activates the c-Jun N-terminal kinase signaling pathway.

PMID: 12612079 by Wright J.H., et al. The STE20 kinase HGK is broadly expressed in human tumor cells and can modulate cellular transformation, invasion, and adhesion.