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- Table of Contents
MRC1 · Western blot design guide
MRC1 Western Blot Planning Guide
Plan a MRC1 Western blot around the catalog-observed 166 kDa band, image-backed A02285-2 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
MRC1 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 166 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Lung | |
| Negative control | Appendix |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 166 kDa |
| ⓘ | Localization | Endosome membrane / Cell membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated MRC1 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Lung |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A02285-2 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected MRC1 Western Blot Band Size?
Use the product-observed 166 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 166 kDa | Matches the authoritative product WB observation. |
| 166 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected MRC1 appearancePlan around 166 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 166 kDa; use this as the primary experimental expectation. |
| Calculated mass | 166 kDa from UniProt P22897; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A02285-2. |
| Situation | Likely cause | Next action |
|---|---|---|
| 166 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing MRC1 band
A02285-2 · MRC1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for MRC1 Western blot
🧪Use Lung as the first positive-control candidate and Appendix as the HPA Not detected negative candidate.
Positive control: Lung (High)
Negative control: Appendix (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for MRC1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Lung | Reported tissue cells | High | Protein (HPA) | HPA → |
| Colon | Reported tissue cells | Low | Protein (HPA) | HPA → |
| Kidney | Reported tissue cells | Low | Protein (HPA) | HPA → |
| Endometrium | Reported tissue cells | Low | Protein (HPA) | HPA → |
Section 3
Advanced MRC1 Western Blot Tips
Deeper troubleshooting and optimisation questions for MRC1, answered from its protein features.
Which band should guide the blot?
Use 166 kDa, the observation attached to the authoritative A02285-2 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 166 kDa expectation.
Which positive control should I start with?
Start with Lung, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Appendix as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for MRC1 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A02285-2 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
MRC1 Western Blot Reagents
Human/Mouse/Rat-reactive MRC1 Western blot reagents with authoritative product imagery.
Real WB data
Anti-Mannose Receptor/MRC1 Picoband® Antibody
Cat # A02285-2
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P22897
- Human Protein Atlas — MRC1
- A02285-2 product record
- PMC7577715 — Natural polymorphism of Ym1 regulates pneumonitis through alternative activation of macrophages (Science advances, 2020)