MSI1 Antibodies

RNA-binding protein Musashi homolog 1 (MSI1)

RNA binding protein that regulates the expression of target mRNAs in the translation level. Regulates expression of this NOTCH1 antagonist NUMB.

Binds RNA containing the sequence 5'- GUUAGUUAGUUAGUU-3' along with other sequences containing the pattern 5'- [GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).

Gene Information

Human
Gene Name:	MSI1
Uniprot:	O43347
Entrez:	4440

Family

Belongs to:
Musashi family

Alternative Names

MSI1; Musashi (Drosophila) homolog 1; musashi homolog 1 (Drosophila); Musashi1; Musashi-1; RNA-binding protein Musashi homolog 1

Molecular Weight

Mass (kDA):
39.125 kDA

Genomic Context

Human
Location:	12q24.31
Sequence:	12; NC_000012.12 (120339662..120369174, complement)

Tissue Specificity

Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.

Subcellular Localizations

Cytoplasm. Nucleus.

Diseases

• Neoplasms
• Malignant Neoplasms
• Nervousness
• Brain Neoplasms
• Cell Transformation, Neoplastic
• Glioma
• Carcinoma
• Malignant Paraganglionic Neoplasm
• Glioblastoma
• Medulloblastoma
• Retinoblastoma
• Neoplasm Metastasis
• Adenocarcinoma

• Colorectal Cancer
• Colorectal Neoplasms
• Dysplasia
• Multiple Endocrine Neoplasia
• Carcinogenesis
• Ischemia
• Neurodegenerative Disorders

Pathways

• Cell Proliferation
• Chromatin Assembly
• Neurogenesis
• Cell Differentiation
• Translation
• Cell Cycle
• Localization
• Fertilization
• Cell Division
• System Development
• Methylation
• Nervous System Development
• Stem Cell Maintenance

• Asymmetric Cell Division
• Seed Development
• Mitosis
• Endosperm Development
• Regeneration
• Pathogenesis
• Virulence

PTMs

• Methylation
• Phosphorylation
• Acetylation
• Demethylation

• Ubiquitination
• Reduction
• Glycosylation
• Ribosylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/MSI1

Best Uses Of The MSI1 Marker

Boster Bio: MSI1 is a gene encoding an important cell adhesion molecule known as mucin. This marker can be found in the cells of the gut. Its overexpression is associated with colon cancer. This article will explain how MSI1 can cause colon cancer. MSI2 Knockdown, which can be used to reduce the expression, is also discussed.

MSI1

The MSI1 marker's best use is in cancer research. It is believed that it inhibits Tensin3's translation and promotes tumor progression. The gene also produces Intercellular Adhesion Molecule-1. This is a factor which contributes to cell movement. MSI1 has been shown to promote tumor progression via its interaction with Ago2, which recruits into the cytosol under hypoxic stress. The complex binds directly to mRNAs important for cell cycle regulation. It also destabilizes mRNAs that affect apoptosis. Its function promotes tumor progression via stress-induced cell growth.

The MSI1 gene encodes a protein that has two tandem copies. It is highly expressed within neural and CNS stem cell populations in vertebrates. It has three binding sites that can be used to bind to the 3'UTR. It is a highly conserved RNA recognition motif that consists of an antiparallel b-sheet packed against two a-helices. The motif repeats twice to three times in 3'UTR.

In a previous study, researchers found that over-expression of Msi1 in the intestinal epithelium decreased the proliferation of these cells. The intestinal length was also reduced by Msi1 expression. The growth of crypt-villi was also reduced. This gene is vital in many aspects relating to intestinal function. Research is ongoing to uncover its best uses. What are the best uses and benefits of the MSI1 gene?

In the mouse knock-in model, the MSI1 gene was found. The transgene was inserted into the Rosa26 locus, which contains a F3/FRT-RMCE docking site. MSI1 has been found to have a high level of gene expression in these mice, according to molecular analysis. After DAPI staining of the tissues, this gene could be detected. While there is still much to do, we have seen some promising signs.

It is well known that Msi1 regulates differentiation of intestinal stem cells in both a temporal- and region-specific fashion. It regulates Notch activity in certain regions. The research also suggests that the gene might be responsible for intestinal defects. Recent research focuses on its effects on intestinal biology. This gene can be used in many different areas of disease research.

Overexpression of MSI1

The MSI1 protein is an RNA binding protein that plays multiple roles in cancer. It regulates p21 expression, and is highly expressed in breast and prostate cancers. The tumor's differentiation, lymphode invasion and distant metastasis are all affected by MSI1. It is also an attractive therapeutic target. However, MSI1's utility as a diagnostic tool is still unknown.

Boster bio: MSI1 was recently used to detect changes within the expression of EMT proteins in transwell experiments. These proteins are less expressed by the shMSI1 marker. MSI2 knockdown decreases invasion and migration and represses other EMT-related proteins. It reverses the expression of EMT-related protein, which in turn inhibits cell invasion and migration.

Multiple studies have shown MSI1 to increase expression of stemness marker CD44. One study used RNA co-immunoprecipitation to find that MSI1 mRNA is associated with CD44 in gliomas. MSI1 was also found associate with the GBM CSC Marker CD44. The MSI1 genome has many applications in cancer research.

It has been demonstrated that the MSI1 gene is important for GBM-derived cell types. It is believed to inhibit pro-neural cell differentiation and promote self regeneration in these cells. MSI1 is also regulated in neural cells under neuronal stem cell conditions. MSI2 might be a potential target for an antimetastatic therapy for human NF1–MPNST.

The MSI1 gene is one the most closely regulated genes in cancer research. It regulates the expression and metabolism of CD44 mRNA. MSI1 binds to U251MG cells preferentially to 3'UTR, but is inactive in KNS42 cell. The three-UTR luciferase reporter monitored MSI1's activity.

Multiple studies have shown the importance of MSI1 for cancer research. For example, the MSI2/CAV1-signaling-pathway plays a role in the metastasis of NF1-MPNST. However, it is not clear how its regulation works. The MSI2 marker is also associated sporadic MPNST with a poorer outcome.

MSI2 knockdown

The MSI1 gene is a RNA-binding protein that regulates cell proliferation and the Wnt/Notch signaling pathways. Overexpression of MSI1 results in an increase in the proliferation of cancer-related stem cells, which is a hallmark of many types. In addition to its role in tumor growth, MSI1 suppresses Akt and p21Cip1 signaling pathways. MSI1 is therefore a great candidate for cancer therapy.

The MSI1 gene was initially discovered in eukaryotic cells in the early 1980s. It was identified in many different cancers, and other diseases. Boster Bio now sells this gene as an anti-bodicle. It is used to diagnose and test biological processes. MSI1 is an essential component of the immune system. It has many applications in human-cancer research. However, MSI1's toxicity has limited its application.

It has also been used in the identification of GBM stem and function cells involved in tumor development. It has been demonstrated that HAL8 tumorspheres and GBM tumorspheres can be made less viable by deleting the MSI1 gene. MSI1 also showed strong co-expression with GBM stem cell markers. MSI1 knockdown also impairs the growth and survival of tumor cells. This marker is vital for understanding how cancer cell responses to chemotherapy.

Additionally, silencing MSI1 genes increases the frequency of NHEJ repairs in cancer cells. It also decreases the activity of proteasome pathways and increases the amount of ubiquitinated substances. Multiple corresponding bands were detected in immunoprecipitation tests of the anti MSI1 antibody. In MSI1 knockout mice, ubiquitination is increased and tumor cells are more easily destroyed by proteasomal degrading agents.

In both HAL8- and KNS42 cells, the RNA binding MSI1 inhibited CD44's turnover. It also inhibited the expression nuMB mRNA from both cell lines. However, the expression of MSI1 in the absence of nuclei of CD44 was unaffected. This shows that MSI1 mRNA interacts well with both miR27b and MIR-143.

MSI1 overexpression is linked to colon cancer

To identify MSI1-positive colon carcinoma cells, it is important to look at the markers of cytokines. These cytokines are metabolized in the pancreas. MSI1 expression is found in both cancer cells as well as healthy colon cells. However it was not clear if this expression was related with the anatomical location or the type of cancer. However, it was discovered that MSI1 is a useful marker of colon cancer.

A favorable prognosis is associated with high MSI1 levels in colon carcinoma. It has been linked with a high degree of local inflammation infiltrate and a large proportion T-lymphocytes within tumors. These findings suggest that colon and systemic inflammation are linked to cancer. However, the underlying mechanisms remain elusive. MSI1 levels, along with other markers, may be used to help patients with poorer prognoses and increase their chances for survival.

Despite low MSI1 levels MSI1 positive cells have high amounts of cancer stem cells (CSC), which are responsible for recurrences as well as treatment resistance. Silibinin can block the CSC-associated transcription proteins and could eradicate colon cancer. Furthermore, it may also be useful for other cancers in the body, including those that harbor CSC.

The MSI1 marker has been shown to correlate with Fn-positive early-stage colorectal cancers. Fn-DNA levels in CRC patients with early-stage disease showed higher levels than those with advanced stages. The anti-Fn levels of early-stage CRC patients were similar, which may indicate an early diagnostic value.