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- Table of Contents
Designing a Western blot for PER2? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the PER2 Western Blot GuideFacts about Period circadian protein homolog 2.
It's derived from the Latin roots'circa' (about) and'diem' (day) and acts as an important regulator of a wide array of physiological functions such as metabolism, sleep, body temperature, blood pressure, endocrine, immune, cardiovascular, and renal function. Consists of two major elements: the central clock, residing in the suprachiasmatic nucleus (SCN) of the brain, along with the peripheral clocks which are found in nearly every tissue and organ system.
| Human | |
|---|---|
| Gene Name: | PER2 |
| Uniprot: | O15055 |
| Entrez: | 8864 |

| Belongs to: |
|---|
| No superfamily |

Circadian clock protein PERIOD 2; FASPS; hPER2; KIAA0347period 2; period (Drosophila) homolog 2; period circadian protein 2; period circadian protein homolog 2; period homolog 2 (Drosophila)
Mass (kDA):
136.579 kDA

| Human | |
|---|---|
| Location: | 2q37.3 |
| Sequence: | 2; NC_000002.12 (238244038..238290102, complement) |
Widely expressed. Found in heart, brain, placenta, lung, liver, skeleatal muscle, kidney and pancreas. High levels in skeletal muscle and pancreas. Low levels in lung. Isoform 2 is expressed in keratinocytes (at protein level).
[Isoform 1]: Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Translocate to the nucleus after phosphorylation by CSNK1D or CSNK1E. Also translocated to the nucleus by CRY1 or CRY2. PML regulates its nuclear localization.; [Isoform 2]: Nucleus, nucleolus.






PMID: 11165242 by Camacho F., et al. Human casein kinase Idelta phosphorylation of human circadian clock proteins period 1 and 2.
PMID: 9427249 by Shearman L.P., et al. Two period homologs: circadian expression and photic regulation in the suprachiasmatic nuclei.