This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
DNA polymerase eta Antibodies
AND POLH ELISA kits, DNA polymerase eta Proteins
Many biological assays use antibodies to detect DNA polymerase eta, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with DNA polymerase eta in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop DNA polymerase eta antibodies...
Designing a Western blot for DNA polymerase eta? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the POLH Western Blot GuidePOLH Infographic by Boster Bio
All POLH Products
Product
Figures
Specificity
Applications
Price
Human, Rat
Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # PB9813
100 μg/vial
Cat # PB9813
100 μg/vial
Human
$960
Cat # LS005429
20 µg, 100 µg
Cat # LS005429
20 µg, 100 µg
POLH Overview
Facts about DNA polymerase eta.
DNA polymerase eta (POLH)
DNA polymerase especially involved in the DNA repair by translesion synthesis (TLS) (PubMed:10385124, PubMed:11743006, PubMed:24449906). As a result of low processivity on both normal and damaged DNA, cooperates with the heterotetrameric (REV3L, REV7, POLD2 and POLD3) POLZ complex for complete bypass of DNA lesions.
Inserts one or 2 nucleotide(s) opposite the lesion, the primer is further extended by the tetrameric POLZ complex. In the case of 1,2-intrastrand d(GpG)-cisplatin cross-link, inserts dCTP opposite the 3' guanine (PubMed:24449906).
Gene Information
| Human | |
|---|---|
| Gene Name: | POLH |
| Uniprot: | Q9Y253 |
| Entrez: | 5429 |
Family
| Belongs to: |
|---|
| DNA polymerase type-Y family |
Alternative Names
DNA polymerase eta; DNA Polymerase, eta; EC 2.7.7.7; POLH; polymerase (DNA directed), eta; RAD30 homolog A; RAD30; RAD30A; RAD30AFLJ16395; Xeroderma pigmentosum variant type protein; XPV; XP-V; XPVFLJ21978
Molecular Weight
Mass (kDA):
78.413 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 6p21.1 |
| Sequence: | 6; NC_000006.12 (43576147..43620523) |
Subcellular Localizations
Nucleus. Binding to ubiquitinated PCNA mediates colocalization to replication foci during DNA replication and persists at sites of stalled replication forks following UV irradiation (PubMed:12606586, PubMed:16357261, PubMed:24553286). After UV irradiation, recruited to DNA damage sites within 1 hour, to a maximum of about 80%; this recruitment may not be not restricted to cells active in DNA replication (PubMed:22801543). Colocalizes with TRAIP to nuclear foci (PubMed:24553286).
Diseases
- Xeroderma Pigmentosum
- Xeroderma
- Malignant Neoplasms
- Malignant Neoplasm Of Skin
- Skin Neoplasms
- Virus Diseases
- Neoplasms
- Carcinogenesis
- Coinfection
- Malignant Squamous Cell Neoplasm
- Carcinoma
- Hereditary Diseases
Interacting Proteins
- BRCA2
- PALB2
- UBC
Pathways
- Translesion Synthesis
- Dna Repair
- Dna Replication
- Virulence
- Translation
- S Phase
- Proteolysis
- Somatic Hypermutation Of Immunoglobulin Genes
- Localization
- Mismatch Repair
- Rna Interference
- Cell Cycle
- Cell Death
PTMs
- Cleavage
- Phosphorylation
- Deamination
Research Areas
- DNA Polymerases
- DNA Repair
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/POLH
bosterbio.com/bosterbio-gene-info-cards/POLH
References
PMID: 10385124 by Masutani C., et al. The XPV (Xeroderma pigmentosum variant) gene encodes human DNA polymerase eta.
PMID: 10398605 by Johnson R.E., et al. hRAD30 mutations in the variant form of xeroderma pigmentosum.