This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
POSTN · Western blot design guide
POSTN Western Blot Planning Guide
Plan a POSTN Western blot around the catalog-observed 93.3 kDa band, image-backed PA2048 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
POSTN Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 93.3 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Breast | |
| Negative control | Appendix |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 93.3 kDa |
| ⓘ | Localization | Golgi apparatus / Secreted |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Mouse / Rat |
Section 1
Real Curated POSTN Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Breast |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PA2048 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected POSTN Western Blot Band Size?
Use the product-observed 93.3 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 93.3 kDa | Matches the authoritative product WB observation. |
| 93.3 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected POSTN appearancePlan around 93.3 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 93.3 kDa; use this as the primary experimental expectation. |
| Calculated mass | 93.3 kDa from UniProt Q15063; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PA2048. |
| Situation | Likely cause | Next action |
|---|---|---|
| 93.3 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing POSTN band
PA2048 · POSTN
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for POSTN Western blot
🧪Use Breast as the first positive-control candidate and Appendix as the HPA Not detected negative candidate.
Positive control: Breast (High)
Negative control: Appendix (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for POSTN
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Breast | Reported tissue cells | High | Protein (HPA) | HPA → |
| Fallopian tube | Reported tissue cells | High | Protein (HPA) | HPA → |
| Lung | Reported tissue cells | High | Protein (HPA) | HPA → |
| Bronchus | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 3
Advanced POSTN Western Blot Tips
Deeper troubleshooting and optimisation questions for POSTN, answered from its protein features.
Which band should guide the blot?
Use 93.3 kDa, the observation attached to the authoritative PA2048 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 93.3 kDa expectation.
Which positive control should I start with?
Start with Breast, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Appendix as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for POSTN Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PA2048 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
POSTN Western Blot Reagents
Mouse/Rat-reactive POSTN Western blot reagents with authoritative product imagery.
Real WB data
Anti-Periostin/POSTN Antibody Picoband®
Cat # PA2048
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q15063
- Human Protein Atlas — POSTN
- PA2048 product record
- PMC13499037 — Scleral Postn Drives Myopia via Promoting Myofibroblast Transdifferentiation (Investigative ophthalmology & visual science, 2026)
- PMC9319689 — DDR2 Expression in Cancer-Associated Fibroblasts Promotes Ovarian Cancer Tumor Invasion and Metastasis through Periostin-ITGB1 (Cancers, 2022)
- PMC11543875 — Periostin regulates the activity of keloid fibroblasts by activating the JAK/STAT signaling pathway (Heliyon, 2024)
