|Validated Species:||Mouse, Rat|
Data & Images
|Product Name||Anti-Periostin/OSF2 Antibody|
|Description||Rabbit IgG polyclonal antibody for Periostin(POSTN) detection. Tested with WB in Mouse;Rat.|
|Cite This Product||Anti-Periostin/OSF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2048)|
|Replacement Item||This antibody may replace the following items: sc-398631|sc-49479|sc-49480|sc-67233 from Santa Cruz Biotechnology.|
|Validated Species||Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of mouse Periostin(24-41aa NSYYDKVLAHSRIRGRDQ), identical to the related rat sequence, and different from the related human sequence by three amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||93144 MW|
|Protein Function||Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX. .|
|Tissue Specificity||Preferentially expressed in periosteum and periodontal ligament. Also expressed in the developing and adult heart. .|
|Sequence Similarities||Contains 1 EMI domain.|
|Subcellular Localization||Golgi apparatus. Secreted, extracellular space, extracellular matrix. Colocalizes with BMP1 in the Golgi.|
|Alternative Names||Periostin;PN;Osteoblast-specific factor 2;OSF-2;Postn;Osf2;|
|Research Areas|||signal transduction|cytoskeleton / ecm|extracellular matrix|structures|focal adhesions| signal transduction|bone| tags & cell markers|cell type markers|other cell types| cancer|invasion/microenvironment| cardiovascular|atherosclerosis|vascular inflammation|leukocyte recruitment|cell adhesion molecules|heart|cardiogenesis|cardiomyocyte precursors||
Background for Periostin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Periostin/OSF2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Mouse, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Periostin/OSF2 Antibody Images
Click the images to enlarge.
All lanes: Anti Periostin (PA2048) at 0.5ug/ml
WB: Mouse Cardiac Muscle Tissue Lysate at 50ug
Predicted bind size: 93KD
Observed bind size: 93KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,