Anti-Periostin/POSTN Antibody Picoband®

Product Name

Anti-Periostin/POSTN Antibody Picoband®

View all Postn Antibodies

SKU/Catalog Number

PA2048

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Periostin/POSTN Antibody catalog # PA2048. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

POSTN (Periostin) is described with evidence that recombinant periostin supports adhesion of ovarian epithelial cells and that periostin is overexpressed in many examined primary breast cancers; tumor-cell periostin overexpression is reported to accelerate xenograft growth and angiogenesis, and extracellular periostin is reported to induce cardiomyocyte cell-cycle reentry. Assay context: antibody validated for WB; reactive to Postn in mouse/rat; immunogen is an N-terminus peptide (mouse; identical in rat) with reported observed MW ~93 kDa and stated no cross-reactivity. Frequently contextualized with vascular markers such as CD34 (angiogenesis) and fibrillar matrix proteins like COL1A1 (ECM remodeling; putative); spatial localization questions are often addressed by IHC in tissue sections (putative, noting this product’s validation list is WB).

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Periostin/POSTN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2048)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of mouse Periostin, identical to the related rat sequence, and different from the related human sequence by three amino acids.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2048 is reactive to Postn in Mouse, Rat

Observed Molecular Weight

93 kDa

Calculated molecular weight

93.1 kDa

Background of Postn

POSTN (Periostin), also known as PN or OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Gillan et al. (2002) found that purified recombinant PN supported adhesion of ovarian epithelial cells. Shao et al. (2004) found that periostin was overexpressed by the majority of human primary breast cancers examined. Transfected tumor cell lines overexpressing periostin showed accelerated growth and angiogenesis as xenografts in immunocompromised animals. Kuhn et al. (2007) showed that extracellular periostin induced reentry of differentiated mammalian cardiomyocytes into the cell cycle.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2048 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat

Positive Control

WB: Mouse Cardiac Muscle Tissue

Validation Images & Assay Conditions

See full target information Postn

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