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- Table of Contents
PRKCB · Western blot design guide
PRKCB Western Blot Planning Guide
Plan a PRKCB Western blot around the catalog-observed 76.9 kDa band, image-backed A01940 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
PRKCB Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 76.9 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Spleen | |
| Negative control | Bone marrow |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 76.9 kDa |
| ⓘ | Localization | Cytoplasm / Nucleus |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated PRKCB Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Lymph node |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A01940 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected PRKCB Western Blot Band Size?
Use the product-observed 76.9 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 76.9 kDa | Matches the authoritative product WB observation. |
| 76.9 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected PRKCB appearancePlan around 76.9 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 76.9 kDa; use this as the primary experimental expectation. |
| Calculated mass | 76.9 kDa from UniProt P05771; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A01940. |
| Situation | Likely cause | Next action |
|---|---|---|
| 76.9 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing PRKCB band
A01940 · PRKCB
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for PRKCB Western blot
🧪Use Spleen as the first positive-control candidate and Bone marrow as the HPA Not detected negative candidate.
Positive control: Spleen (High)
Negative control: Bone marrow (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for PRKCB
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Spleen | Reported tissue cells | High | Protein (HPA) | HPA → |
| Lymph node | Reported tissue cells | High | Protein (HPA) | HPA → |
| Tonsil | Reported tissue cells | High | Protein (HPA) | HPA → |
| Caudate | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 3
Advanced PRKCB Western Blot Tips
Deeper troubleshooting and optimisation questions for PRKCB, answered from its protein features.
Which band should guide the blot?
Use 76.9 kDa, the observation attached to the authoritative A01940 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 76.9 kDa expectation.
Which positive control should I start with?
Start with Spleen, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Bone marrow as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for PRKCB Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A01940 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
PRKCB Western Blot Reagents
Human/Mouse/Rat-reactive PRKCB Western blot reagents with authoritative product imagery.
Real WB data
Anti-PKC beta 1/PRKCB Antibody Picoband®
Cat # A01940
Real WB data
Anti-PKC beta 1/PRKCB Antibody Picoband®
Cat # PB9319
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P05771
- Human Protein Atlas — PRKCB
- A01940 product record
- PMC13462150 — Adenylate cyclase 4 suppresses malignant progression of lung adenocarcinoma through interaction with protein kinase C beta (Translational cancer research, 2026)
- PMC5085156 — Identification of miR-34a-target interactions by a combined network based and experimental approach (Oncotarget, 2016)
- PMC6472018 — Decreased Protein Kinase C-β Type II Associated with the Prominent Endotoxin Exhaustion in the Macrophage of FcGRIIb-/- Lupus Prone Mice is Revealed by Phosphoproteomic Analysis (International journal of molecular sciences, 2019)