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- Table of Contents
PTH1R · Western blot design guide
PTH1R Western Blot Planning Guide
Plan a PTH1R Western blot around the catalog-observed 66.4 kDa band, image-backed A01588 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
PTH1R Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 66.4 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Bronchus | |
| Negative control | Appendix |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 66.4 kDa |
| ⓘ | Localization | Cell membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated PTH1R Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Bronchus |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A01588 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected PTH1R Western Blot Band Size?
Use the product-observed 66.4 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 66.4 kDa | Matches the authoritative product WB observation. |
| 66.4 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected PTH1R appearancePlan around 66.4 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 66.4 kDa; use this as the primary experimental expectation. |
| Calculated mass | 66.4 kDa from UniProt Q03431; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A01588. |
| Situation | Likely cause | Next action |
|---|---|---|
| 66.4 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing PTH1R band
A01588 · PTH1R
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for PTH1R Western blot
🧪Use Bronchus as the first positive-control candidate and Appendix as the HPA Not detected negative candidate.
Positive control: Bronchus (High)
Negative control: Appendix (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for PTH1R
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Bronchus | Reported tissue cells | High | Protein (HPA) | HPA → |
| Kidney | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Fallopian tube | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Nasopharynx | Reported tissue cells | Low | Protein (HPA) | HPA → |
Section 3
Advanced PTH1R Western Blot Tips
Deeper troubleshooting and optimisation questions for PTH1R, answered from its protein features.
Which band should guide the blot?
Use 66.4 kDa, the observation attached to the authoritative A01588 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 66.4 kDa expectation.
Which positive control should I start with?
Start with Bronchus, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Appendix as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for PTH1R Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A01588 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
PTH1R Western Blot Reagents
Human/Mouse/Rat-reactive PTH1R Western blot reagents with authoritative product imagery.
Real WB data
Anti-Parathyroid Hormone Receptor 1/PTH1R Antibody Picoband®
Cat # A01588
Real WB data
Anti-Parathyroid Hormone Receptor 1/PTH1R Antibody Picoband®
Cat # PA2132
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q03431
- Human Protein Atlas — PTH1R
- A01588 product record
- PMC7525296 — CHD7 Regulates Osteogenic Differentiation of Human Dental Follicle Cells via PTH1R Signaling (Stem cells international, 2020)
- PMC3490049 — Fluorescent ligand-directed co-localization of the parathyroid hormone 1 receptor with the brush-border scaffold complex of the proximal tubule reveals hormone-dependent changes in ezrin immunoreactivity consistent with inactivation (Biochimica et biophysica acta, 2012)
- PMC5665616 — Dual roles of parathyroid hormone related protein in TGF-β1 signaling and fibronectin up-regulation in mesangial cells (Bioscience reports, 2017)