Product Info Summary
| SKU: | PA2132 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-Parathyroid Hormone Receptor 1/PTH1R Antibody Picoband®
SKU/Catalog Number
PA2132
BA3170 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Parathyroid Hormone Receptor 1/PTH1R Antibody catalog # PA2132. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Parathyroid Hormone Receptor 1/PTH1R Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2132)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Parathyroid Hormone Receptor 1, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2132 is reactive to PTH1R in Human, Mouse, Rat
Observed Molecular Weight
66 kDa
Calculated molecular weight
66.4 kDa
Background of PTH1R
Parathyroid hormone/parathyroid hormone-related peptide receptor, also known as PTH1R, PTHR, is a protein that in humans is encoded by the PTH1R gene. PTHR is a member of a family of G protein-coupled receptors. PTHR gene was mapped to the vicinity of the 3p21.3-p21.2 boundary by PCR analysis of human/rodent somatic cell hybrid panels using oligonucleotide primers designed to amplify a portion of the gene from genomic DNA. This is a receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. PTH1R functions as a receptor for parathyroid hormone and for parathyroid hormone-related protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2132 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: SKOV Whole Cell, U20S Whole Cell, HELA Whole Cell, SMMC Whole Cell
IHC: Human Thyroid Cancer Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-Parathyroid Hormone Receptor 1 antibody, PA2132, Western blotting
All lanes: Anti Parathyroid Hormone Receptor 1 (PA2132) at 0.5ug/ml
Lane 1: SKOV Whole Cell Lysate at 40ug
Lane 2: U20S Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 66KD
Observed bind size: 66KD
Click image to see more details
Anti-Parathyroid Hormone Receptor 1 antibody, PA2132, IHC(P)
IHC(P): Human Thyroid Cancer Tissue
Specific Publications For Anti-Parathyroid Hormone Receptor 1/PTH1R Antibody Picoband® (PA2132)
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Customer Q&As
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4 Customer Q&As for Anti-Parathyroid Hormone Receptor 1/PTH1R Antibody Picoband®
Question
I see that the anti-Parathyroid Hormone Receptor 1/PTH1R antibody PA2132 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-12-12
Question
Is a blocking peptide available for product anti-Parathyroid Hormone Receptor 1/PTH1R antibody (PA2132)?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
We do provide the blocking peptide for product anti-Parathyroid Hormone Receptor 1/PTH1R antibody (PA2132). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-07-01
Question
Is this PA2132 anti-Parathyroid Hormone Receptor 1/PTH1R antibody reactive to the isotypes of PTH1R?
Verified Customer
Verified customer
Asked: 2018-08-23
Answer
The immunogen of PA2132 anti-Parathyroid Hormone Receptor 1/PTH1R antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Parathyroid Hormone Receptor 1(388-406aa KLRETNAGRCDTRQQYRKL), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-23
Question
We are currently using anti-Parathyroid Hormone Receptor 1/PTH1R antibody PA2132 for mouse tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2017-12-14
Answer
The anti-Parathyroid Hormone Receptor 1/PTH1R antibody (PA2132) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-14


