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PHF8 Antibodies
AND PHF8 ELISA kits, PHF8 Proteins
Many biological assays use antibodies to detect PHF8, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with PHF8 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop PHF8 antibodies...
Designing a Western blot for PHF8? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the PHF8 Western Blot GuideDesigning an IHC experiment for PHF8? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the PHF8 IHC GuidePHF8 Infographic by Boster Bio
All PHF8 Products
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Specificity
Applications
Price
PHF8 Overview
Facts about Histone lysine demethylase PHF8.
Histone lysine demethylase PHF8 (PHF8)
Histone lysine demethylase with selectivity for the di- and monomethyl conditions that plays an integral role cell cycle progression, rDNA transcription and brain growth. Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks.
Involved in cell cycle progression by being required to restrain G1-S transition. Acts as a coactivator of rDNA transcription, by triggering polymerase I (pol I) mediated transcription of rRNA genes.
Gene Information
| Human | |
|---|---|
| Gene Name: | PHF8 |
| Uniprot: | Q9UPP1 |
| Entrez: | 23133 |
Family
| Belongs to: |
|---|
| JHDM1 histone demethylase family |
Alternative Names
EC 1.14.11.27; histone lysine demethylase PHF8; jumonji C domain-containing histone demethylase 1F; KIAA1111ZNF422JHDM1F; MRXSSD; PHD finger protein 8DKFZp686E0868
Molecular Weight
Mass (kDA):
117.864 kDA
Genomic Context
| Human | |
|---|---|
| Location: | Xp11.22 |
| Sequence: | X; NC_000023.11 (53936680..54048935, complement) |
Subcellular Localizations
Nucleus. Nucleus, nucleolus. Recruited to H3K4me3 sites on chromatin during interphase. Dissociates from chromatin when cells enter mitosis.
Diseases
- Cleft Lip
- Cleft Palate
- Malignant Neoplasms
- Bilateral Cleft Lip
- Cell Invasion
- Malignant Neoplasm Of Prostate
- Congenital Abnormality
- Autistic Disorder
- Prostatic Neoplasms
- Autism Spectrum Disorders
- Neoplasm Metastasis
Interacting Proteins
- RARA
- RBPJ
Pathways
- Methylation
- Demethylation
- Histone Methylation
- Histone Demethylation
- Cell Cycle
- Pathogenesis
- Cell Adhesion
- Cell Migration
- Cell Proliferation
- Gene Silencing
- Cell Development
- Mitosis
- Dna Methylation
PTMs
- Methylation
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PHF8
bosterbio.com/bosterbio-gene-info-cards/PHF8
References
PMID: 16199551 by Laumonnier F., et al. Mutations in PHF8 are associated with X linked mental retardation and cleft lip/cleft palate.
PMID: 20531378 by Zhu Z., et al. PHF8 is a histone H3K9me2 demethylase regulating rRNA synthesis.