DNA endonuclease RBBP8 (RBBP8)

Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double- strand break (DSB) repair through the homologous recombination (HR) pathway. HR is limited to S and G2 phases of the cell cycle and preferentially repairs DSBs caused by replication fork collapse.

Key determinant of DSB repair pathway option, as it commits cells to HR by preventing classical non-homologous end- joining (NHEJ). Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the creation of ssDNA.