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- Table of Contents
RECK · Western blot design guide
RECK Western Blot Planning Guide
Plan a RECK Western blot around the catalog-observed 106.5 kDa band, image-backed A06439-1 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
RECK Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 106.5 kDa observed | |
| Observed band | 106.5 kDa observed | |
| Gel | 8-10% | |
| Positive control | Breast | |
| Negative control | Appendix |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 106.5 kDa |
| ⓘ | Localization | Cell membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated RECK Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Breast |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A06439-1 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected RECK Western Blot Band Size?
Use the product-observed 106.5 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 106.5 kDa | Matches the authoritative product WB observation. |
| 106.5 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected RECK appearancePlan around 106.5 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 106.5 kDa; use this as the primary experimental expectation. |
| Calculated mass | 106.5 kDa from UniProt O95980; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A06439-1. |
| Situation | Likely cause | Next action |
|---|---|---|
| 106.5 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing RECK band
A06439-1 · RECK
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for RECK Western blot
🧪Use Breast as the first positive-control candidate and retain an orthogonal negative control.
Positive control: Breast (High)
Negative control: Appendix (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for RECK
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Breast | Reported tissue cells | High | Protein (HPA) | HPA → |
| Colon | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Cerebellum | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Cerebral cortex | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 3
Advanced RECK Western Blot Tips
Deeper troubleshooting and optimisation questions for RECK, answered from its protein features.
Which band should guide the blot?
Use 106.5 kDa, the observation attached to the authoritative A06439-1 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 106.5 kDa expectation.
Which positive control should I start with?
Start with Breast, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Appendix as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for RECK Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A06439-1 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
RECK Western Blot Reagents
Human/Mouse/Rat-reactive RECK Western blot reagents with authoritative product imagery.
Real WB data
Anti-RECK Antibody Picoband®
Cat # A06439-1
Real WB data
Anti-RECK Antibody Picoband®
Cat # A06439-3
Real WB data
Anti-RECK Antibody Picoband®
Cat # A06439-4
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt O95980
- Human Protein Atlas — RECK
- A06439-1 product record
- PMC6881977 — Modulation of RECK levels in Xenopus A6 cells: effects on MT1-MMP, MMP-2 and pERK levels (Journal of biological research (Thessalonike, Greece), 2019)
- PMC5435722 — Nimbolide upregulates RECK by targeting miR-21 and HIF-1α in cell lines and in a hamster oral carcinogenesis model (Scientific reports, 2017)
- PMC3972504 — E-cadherin-downregulation and RECK-upregulation are coupled in the non-malignant epithelial cell line MCF10A but not in multiple carcinoma-derived cell lines (Scientific reports, 2014)