Anti-RECK Antibody Picoband™

Product Name

Anti-RECK Antibody Picoband™

View all RECK Antibodies

SKU/Catalog Number

A06439-4

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-RECK Antibody Picoband™ catalog # A06439-4. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-RECK Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06439-4)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived human RECK recombinant protein (Position: Q179-K806). Human RECK shares 94.3% amino acid (aa) sequence identity with mouse RECK.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A06439-4 is reactive to RECK in Human, Mouse, Rat

Applications

A06439-4 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

100 kDa

Calculated molecular weight

106.457kDa

Background of RECK

Reversion-inducing-cysteine-rich protein with kazal motifs, also known as RECK, is a human gene, thought to be a metastasis suppressor. The protein encoded by this gene is a cysteine-rich, extracellular protein with protease inhibitor-like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane-anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase-9, a key enzyme involved in tumor invasion and metastasis. Several transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of RECK using anti-RECK antibody (A06439-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse LLC whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RECK antigen affinity purified polyclonal antibody (Catalog # A06439-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RECK at approximately 100 kDa. The expected band size for RECK is at 106 kDa.

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Figure 2. Flow Cytometry analysis of JK cells using anti-RECK antibody (A06439-4).
Overlay histogram showing JK cells stained with A06439-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-RECK Antibody (A06439-4, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For RECK (Source: Uniprot.org, NCBI)

Alternative Names

hRECK; membrane-anchored glycoprotein (metastasis and invasion); RECK; reversion-inducing-cysteine-rich protein with kazal motifs; ST15; ST15reversion-inducing cysteine-rich protein with Kazal motifs; suppression of tumorigenicity 15 (reversion-inducing-cysteine-rich protein withkazal motifs); suppression of tumorigenicity 5 (reversion-inducing-cysteine-rich protein withkazal motifs); Suppressor of tumorigenicity 15 protein RECK ST15 reversion inducing cysteine rich protein with kazal motifs reversion-inducing cysteine-rich protein with Kazal motifs|membrane-anchored glycoprotein (metastasis and invasion)|suppression of tumorigenicity 15 (reversion-inducing-cysteine-rich protein with kazal motifs)|suppression of tumorigenicity 5 (reversion-inducing-cysteine-rich protein with kazal motifs)|suppressor of tumorigenicity 15 protein

For more info on RECK, check out the RECK Infographic

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In this infographic, you will see the following information for RECK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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