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- Table of Contents
RPGR · Western blot design guide
RPGR Western Blot Planning Guide
Plan a RPGR Western blot around the catalog-observed 113.4 kDa band, image-backed A01522 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
RPGR Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 113.4 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Fallopian tube | |
| Negative control | Bone marrow |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 113.4 kDa |
| ⓘ | Localization | Cytoplasm, cytoskeleton, flagellum axoneme / Golgi apparatus |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Rat |
Section 1
Real Curated RPGR Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Adrenal gland |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A01522 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected RPGR Western Blot Band Size?
Use the product-observed 113.4 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 113.4 kDa | Matches the authoritative product WB observation. |
| 113.4 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected RPGR appearancePlan around 113.4 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 113.4 kDa; use this as the primary experimental expectation. |
| Calculated mass | 113.4 kDa from UniProt Q92834; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A01522. |
| Situation | Likely cause | Next action |
|---|---|---|
| 113.4 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing RPGR band
A01522 · RPGR
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for RPGR Western blot
🧪Use Fallopian tube as the first positive-control candidate and Bone marrow as the HPA Not detected negative candidate.
Positive control: Fallopian tube (High)
Negative control: Bone marrow (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for RPGR
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Fallopian tube | Reported tissue cells | High | Protein (HPA) | HPA → |
| Gallbladder | Reported tissue cells | High | Protein (HPA) | HPA → |
| Kidney | Reported tissue cells | High | Protein (HPA) | HPA → |
| Adrenal gland | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 3
Advanced RPGR Western Blot Tips
Deeper troubleshooting and optimisation questions for RPGR, answered from its protein features.
Which band should guide the blot?
Use 113.4 kDa, the observation attached to the authoritative A01522 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 113.4 kDa expectation.
Which positive control should I start with?
Start with Fallopian tube, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Bone marrow as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for RPGR Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A01522 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
RPGR Western Blot Reagents
Rat-reactive RPGR Western blot reagents with authoritative product imagery.
Real WB data
Anti-RPGR Antibody Picoband®
Cat # A01522
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q92834
- Human Protein Atlas — RPGR
- A01522 product record
- PMC2905641 — Interaction of ciliary disease protein retinitis pigmentosa GTPase regulator with nephronophthisis-associated proteins in mammalian retinas (Molecular vision, 2010)
- PMC8037643 — The Major Ciliary Isoforms of RPGR Build Different Interaction Complexes with INPP5E and RPGRIP1L (International journal of molecular sciences, 2021)