RNF220 Antibodies

AND RNF220 ELISA kits, RNF220 Proteins

Many biological assays use antibodies to detect RNF220, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with RNF220 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop RNF220 antibodies...

We validate the specificity of these antibodies to RNF220 by testing them on tissues known to express RNF220 positively and negatively. Browse below to find the RNF220 antibody that suites your experiment. We have 4 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing an IHC experiment for RNF220? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.

Open the RNF220 IHC Guide

RNF220 Infographic by Boster Bio

All RNF220 Products

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Product

Figures

Specificity

Applications

Price

Anti-RNF220 Antibody Picoband®

Western blot analysis of RNF220 using anti-RNF220 antibody (A11818-1)

IHC analysis of RNF220 using anti-RNF220 antibody (A11818-1)

Human, Mouse, Rat

ELISA, Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # A11818-1
100 μg/vial

Anti-RNF220 Antibody

Western blot analysis of extracts of various cell lines, using RNF220 antibody

Human, Mouse, Rat

$405
Cat # A11818
100ul

RNF220 (NM_018150) Human Over-expression Lysate

Human

$628
Cat # LS055182
20 µg, 100 µg

RNF220 (NM_018150) Human Recombinant Protein

Coomassie blue staining of purified RNF220 protein

$1249
Cat # PROTQ5VTB9
20 µg

RNF220 Overview

Facts about E3 ubiquitin-protein ligase RNF220.

RNF220 3D structure. Source: alphafold

E3 ubiquitin-protein ligase RNF220 (RNF220)

E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of SIN3B (By similarity). Independently of its E3 ligase activity, acts as a CTNNB1 stabilizer through USP7-mediated deubiquitination of CTNNB1 boosting Wnt signaling (PubMed:25266658).

Gene Information

Human
Gene Name:	RNF220
Uniprot:	Q5VTB9
Entrez:	55182

Family

Belongs to:

No superfamily

Alternative Names

C1orf164; chromosome 1 open reading frame 164; E3 ubiquitin-protein ligase RNF220; EC 6.3.2.-; ring finger protein 220FLJ10597

Molecular Weight

Mass (kDA):
62.765 kDA

Genomic Context


Human

Location:	1p34.1
Sequence:	1; NC_000001.11 (44405160..44651724)

Subcellular Localizations

Cytoplasm.

PTMs

Ubiquitination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/RNF220

References

PMID: 25266658 by Ma P., et al. The ubiquitin ligase RNF220 enhances canonical Wnt signaling through USP7-mediated deubiquitination of beta-catenin.