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- Table of Contents
SAE1 Antibodies
AND SAE1 ELISA kits, SAE1 Proteins
Many biological assays use antibodies to detect SAE1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with SAE1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop SAE1 antibodies...
Designing an IHC experiment for SAE1? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the SAE1 IHC GuideSAE1 Infographic by Boster Bio
All SAE1 Products
Product
Figures
Specificity
Applications
Price
Human, Mouse, Rat
ELISA, IF, IHC, ICC, WB
$370
Cat # A04753-3
100 μg/vial
Cat # A04753-3
100 μg/vial
Human, Rat
Flow Cytometry, IP, IF, ICC, WB
$370
Cat # M04753-2
100 μl/vial
Cat # M04753-2
100 μl/vial
Human, Mouse, Rat
ELISA
$480
Cat # EKC1031
1 kit, containing one 96-well plate and all necessary reagents
Cat # EKC1031
1 kit, containing one 96-well plate and all necessary reagents
SAE1 Overview
Facts about SUMO-activating enzyme subunit 1.
SUMO-activating enzyme subunit 1 (SAE1)
The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
.
Gene Information
| Human | |
|---|---|
| Gene Name: | SAE1 |
| Uniprot: | Q9UBE0 |
| Entrez: | 10055 |
Family
| Belongs to: |
|---|
| ubiquitin-activating E1 family |
Alternative Names
AOS1; AOS1activator of SUMO1; FLJ3091; HSPC140; SAE1; SUA1; SUA1sentrin/SUMO-activating protein AOS1; SUMO Activating Enzyme E1 (SAE1/UBA2); SUMO-1 activating enzyme E1 N subunit; SUMO1 activating enzyme subunit 1; SUMO-1 activating enzyme subunit 1; SUMO-activating enzyme subunit 1; UBA2; Ubiquitin-like 1-activating enzyme E1A; ubiquitin-like protein SUMO-1 activating enzyme; UBLE1A
Molecular Weight
Mass (kDA):
38.45 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 19q13.32 |
| Sequence: | 19; NC_000019.10 (47130835..47210636) |
Tissue Specificity
Expression level increases during S phase and drops in G2 phase (at protein level).
Subcellular Localizations
Nucleus.
Diseases
- Malignant Neoplasms
- Neoplasms
- Cell Transformation, Neoplastic
- Acute Promyelocytic Leukemia
- Mammary Neoplasms
- Leukemia
- Stomach Neoplasms
- Neoplasm Metastasis
- Glioma
- Neoplasm By Morphology
- Dermatomyositis
- Lung Diseases
Interacting Proteins
- UBA2
Pathways
- Conjugation
- Cell Cycle
- Localization
- Prophase
- Pachytene
- Gene Silencing
- Protein Sumoylation
- M Phase
- Keratinocyte Differentiation
- Sporulation
- Cell Cycle Arrest
- Cell Division
- Oncogenesis
PTMs
- Sumoylation
- Phosphorylation
Research Areas
- Stem Cell Markers
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SAE1
bosterbio.com/bosterbio-gene-info-cards/SAE1
References
PMID: 9920803 by Okuma T., et al. In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2.
PMID: 10217437 by Gong L., et al. Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex.