Product Info Summary
| SKU: | PA2220 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Sumo 1/SUMO1 Antibody Picoband®
SKU/Catalog Number
PA2220
BA3364-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Sumo 1/SUMO1 Antibody catalog # PA2220. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Sumo 1/SUMO1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2220)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Sumo 1, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2220 is reactive to SUMO1 in Human, Mouse, Rat
Observed Molecular Weight
91 kDa
Calculated molecular weight
11.6 kDa
Background of SUMO1
Small ubiquitin-related modifier 1 (SUMO1), also called SMT3C or PIC1 is a protein that in humans is encoded by the SUMO1 gene. This gene is mapped to 2q33.1. This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2220 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Spleen Tissue, Human Placenta Tissue,
Validation Images & Assay Conditions
Click image to see more details
Anti-Sumo 1 antibody, PA2220, Western blotting
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Human Placenta Tissue Lysate
Specific Publications For Anti-Sumo 1/SUMO1 Antibody Picoband® (PA2220)
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4 Customer Q&As for Anti-Sumo 1/SUMO1 Antibody Picoband®
Question
My colleagues were happy with the WB result of your anti-Sumo 1/SUMO1 antibody. However we have seen positive staining in placenta nucleus membrane. nucleus speckle. using this antibody. Is that expected? Could you tell me where is SUMO1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
From literature, placenta does express SUMO1. Generally SUMO1 expresses in nucleus membrane. nucleus speckle. Regarding which tissues have SUMO1 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 14702039
Brain, Pubmed ID: 9119407
Brain, and Placenta, Pubmed ID: 8806687
Cervix carcinoma, Pubmed ID: 17081983, 18691976, 20068231, 20388717
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-10
Question
We want using your anti-Sumo 1/SUMO1 antibody for positive regulation of atpase-coupled calcium transmembrane transporter activity studies. Has this antibody been tested with western blotting on human placenta tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
Thanks for your inquiry. This PA2220 anti-Sumo 1/SUMO1 antibody is validated on rat spleen tissue, tissue lysate, human placenta tissue. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-22
Question
We have observed staining in mouse cervix carcinoma erythroleukemia. Any tips? Is anti-Sumo 1/SUMO1 antibody supposed to stain cervix carcinoma erythroleukemia positively?
N. Patel
Verified customer
Asked: 2018-05-02
Answer
Based on literature cervix carcinoma erythroleukemia does express SUMO1. Based on Uniprot.org, SUMO1 is expressed in caudate nucleus, brain, brain placenta, placenta, colon adenocarcinoma, amygdala, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have SUMO1 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 14702039
Brain, Pubmed ID: 9119407
Brain, and Placenta, Pubmed ID: 8806687
Cervix carcinoma, Pubmed ID: 17081983, 18691976, 20068231, 20388717
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-05-02
Question
We are currently using anti-Sumo 1/SUMO1 antibody PA2220 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-04-09
Answer
The anti-Sumo 1/SUMO1 antibody (PA2220) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-09


