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- Table of Contents
TRPM4 · Western blot design guide
TRPM4 Western Blot Planning Guide
Plan a TRPM4 Western blot around the catalog-observed 134.3 kDa band, image-backed PB9902 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
TRPM4 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 134.3 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 8-10% | |
| Positive control | Colon | |
| Negative control | Epididymis |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 134.3 kDa |
| ⓘ | Localization | Cell membrane / Endoplasmic reticulum |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human |
Section 1
Real Curated TRPM4 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Appendix |
| Gel % | 8-10% |
| Load | 20-30 µg total protein per lane |
| Transfer | Wet/tank, extended transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PB9902 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected TRPM4 Western Blot Band Size?
Use the product-observed 134.3 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 134.3 kDa | Matches the authoritative product WB observation. |
| 134.3 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected TRPM4 appearancePlan around 134.3 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 134.3 kDa; use this as the primary experimental expectation. |
| Calculated mass | 134.3 kDa from UniProt Q8TD43; retain as context. |
| Gel selection | 8-10%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PB9902. |
| Situation | Likely cause | Next action |
|---|---|---|
| 134.3 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing TRPM4 band
PB9902 · TRPM4
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for TRPM4 Western blot
🧪Use Colon as the first positive-control candidate and Epididymis as the HPA Not detected negative candidate.
Positive control: Colon (High)
Negative control: Epididymis (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for TRPM4
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Colon | Reported tissue cells | High | Protein (HPA) | HPA → |
| Prostate | Reported tissue cells | High | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | High | Protein (HPA) | HPA → |
| Duodenum | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 3
Advanced TRPM4 Western Blot Tips
Deeper troubleshooting and optimisation questions for TRPM4, answered from its protein features.
Which band should guide the blot?
Use 134.3 kDa, the observation attached to the authoritative PB9902 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 134.3 kDa expectation.
Which positive control should I start with?
Start with Colon, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Epididymis as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 8-10% consistently across the quick facts, protocol table, and poster.
What transfer method to use for TRPM4 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PB9902 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
TRPM4 Western Blot Reagents
Human-reactive TRPM4 Western blot reagents with authoritative product imagery.
Real WB data
Anti-TRPM4 Antibody Picoband®
Cat # PB9902
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q8TD43
- Human Protein Atlas — TRPM4
- PB9902 product record
- PMC13327340 — Glycosylation of the murine cardiac channel TRPM4 is altered by the pathogenic p.I376T variant (Experimental physiology, 2026)
- PMC11322923 — Acetalax (Oxyphenisatin Acetate, NSC 59687) and Bisacodyl Cause Oncosis in Triple-Negative Breast Cancer Cell Lines by Poisoning the Ion Exchange Membrane Protein TRPM4 (Cancer research communications, 2024)
- PMC5759053 — SUR1-TRPM4 and AQP4 form a heteromultimeric complex that amplifies ion/water osmotic coupling and drives astrocyte swelling (Glia, 2018)
