TRPV4 · Western blot design guide

TRPV4 Western Blot Planning Guide

Plan a TRPV4 Western blot around the catalog-observed 98.3 kDa band, image-backed A00565-2 evidence, HPA controls, and verified protocol records.

Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use

Western blot protocol sheet for TRPV4 (TRPV4): expected band 98.3 kDa, antibody A00565-2, and PMC-cited SDS-PAGE protocol steps — TRPV4 Western blot protocol sheet — expected band 98.3 kDa, antibody A00565-2, controls and PMC citations. Open the full TRPV4 WB guide →

Navigate to: Protocols Band Controls Antibodies

TRPV4 Western Blot Experimental Design Guide

Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.

Must know before running

	Expected band	98.3 kDa
	Observed band	Not reported — verify product WB image
	Gel	8-10%
	Positive control ⓘ	Pancreas
	Negative control ⓘ	Bone marrow

Important caveats

Reasons your observed band may differ from the expected size.

ⓘ	Calculated mass	98.3 kDa
ⓘ	Localization	Cell membrane / Apical cell membrane
ⓘ	Processing / PTM	Record-dependent
ⓘ	Reactivity	Human / Mouse / Rat

Section 1

Real Curated TRPV4 Western Blot Protocols

Start with the molecular-weight rule, then compare verified publication-derived conditions.

Recommended Western blot protocol parameters
Sample / lysate	Adrenal gland
Gel %	8-10%
Load	20-30 µg total protein per lane
Transfer	Semi-dry, standard transfer
Membrane	0.45 µm PVDF
Blocking	5% non-fat milk or 5% BSA in TBST
Primary	A00565-2 at datasheet starting dilution
Primary incubation	Overnight at 4 °C with gentle agitation
Secondary	Species-matched HRP conjugate at validated dilution
Wash	3 × 5 min in TBST
Detection	Chemiluminescent substrate
Exposure	Bracket exposures to avoid saturation

Section 2

What Is the Expected TRPV4 Western Blot Band Size?

Use the product-observed 98.3 kDa band as the primary planning value and retain the UniProt calculated mass as context.

What am I looking at on my blot?
98.3 kDa	Matches the authoritative product WB observation.
98.3 kDa calculated	Use as UniProt context, not as a replacement observed band.
Unexpected additional signal	Do not assign identity without orthogonal positive/negative controls.

💡Expected TRPV4 appearancePlan around 98.3 kDa and keep the calculated mass as supporting context.

How each factor affects band size
Catalog-observed band	98.3 kDa; use this as the primary experimental expectation.
Calculated mass	98.3 kDa from UniProt Q9HBA0; retain as context.
Gel selection	8-10%; shared with the recommended protocol and poster.
Specificity check	Compare the lead HPA positive and negative controls with A00565-2.

Why is my band missing or off?
Situation	Likely cause	Next action
98.3 kDa	Matches the authoritative product WB observation.	Confirm with orthogonal controls and the linked product record.
Additional band	May reflect processing, modification, or non-specific signal.	Run a dilution series and compare positive/negative controls.
Weak signal	Target abundance or transfer may be limiting.	Verify transfer, increase positive-control abundance, and bracket exposure.

Sources: UniProt Q9HBA0 Human Protein Atlas product datasheet (A00565-2)

Experiment evidence

Western blot images showing TRPV4 band

A00565-2 · TRPV4

← swipe / scroll for more images →

Real WB data behind the band-size expectations in this section.

Sample controls for TRPV4 Western blot

🧪Use Pancreas as the first positive-control candidate and Bone marrow as the HPA Not detected negative candidate.

Positive control: Pancreas (High)

Negative control: Bone marrow (Not detected)

HPA protein score determines control status; other expression data is supporting context only.

HPA tissue expression evidence for TRPV4

Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.

Positive expression · recommended positive controls

Tissue	Cell type	Level	Evidence	Source
Pancreas	Reported tissue cells	High	Protein (HPA)	HPA →
Adrenal gland	Reported tissue cells	High	Protein (HPA)	HPA →
Hippocampus	Reported tissue cells	High	Protein (HPA)	HPA →
Cerebral cortex	Reported tissue cells	High	Protein (HPA)	HPA →

Undetected expression · recommended negative controls

Tissue	Cell type	Level	Evidence	Source
Bone marrow	Reported tissue cells	Not detected	Protein (HPA)	HPA →
Epididymis	Reported tissue cells	Not detected	Protein (HPA)	HPA →

Section 3

Advanced TRPV4 Western Blot Tips

Deeper troubleshooting and optimisation questions for TRPV4, answered from its protein features.

Which band should guide the blot?

Use 98.3 kDa, the observation attached to the authoritative A00565-2 WB record.

How should calculated mass be interpreted?

Treat the UniProt calculated mass as context; it does not replace the catalog-observed 98.3 kDa expectation.

Which positive control should I start with?

Start with Pancreas, the lead HPA protein-expression candidate.

Which negative control is defensible?

Use Bone marrow as an orthogonal HPA Not detected candidate.

Which gel should I use?

Use 8-10% consistently across the quick facts, protocol table, and poster.

What transfer method to use for TRPV4 Western blot?

Use the transfer method in the recommended protocol and verify transfer before blocking.

How should A00565-2 be started?

Start at the linked datasheet condition and run a three-point primary-antibody dilution test.

Which publication-derived protocols can I compare?

Compare only the supplied target-verified records: PMC12597633, PMC6731547, PMC9815116.

Boster reagents

TRPV4 Western Blot Reagents

Human/Mouse/Rat-reactive TRPV4 Western blot reagents with authoritative product imagery.

Real WB data Western blot validation image for TRPV4 using A00565-2; observed band 98.3 kDa

Anti-TRPV4 Antibody Picoband®

Cat # A00565-2

View product →

Real WB data Western blot validation image for TRPV4 using A00565-3; observed band 98.3 kDa