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- Table of Contents
UBA3 Antibodies
AND UBA3 ELISA kits, UBA3 Proteins
Many biological assays use antibodies to detect UBA3, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with UBA3 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop UBA3 antibodies...
Designing a Western blot for UBA3? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the UBA3 Western Blot GuideDesigning an IHC experiment for UBA3? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the UBA3 IHC GuideUBA3 Infographic by Boster Bio
All UBA3 Products
Product
Figures
Specificity
Applications
Price
Human, Mouse, Rat
Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # PB9838
100 μg/vial
Cat # PB9838
100 μg/vial
UBA3 Overview
Facts about NEDD8-activating enzyme E1 catalytic subunit.
NEDD8-activating enzyme E1 catalytic subunit (UBA3)
Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, then linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP.
E1 eventually transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity.
Gene Information
| Human | |
|---|---|
| Gene Name: | UBA3 |
| Uniprot: | Q8TBC4 |
| Entrez: | 9039 |
Family
| Belongs to: |
|---|
| ubiquitin-activating E1 family |
Alternative Names
DKFZp566J164; EC 6.3.2; EC 6.3.2.-; hUBA3; MGC22384; NEDD8-activating enzyme E1 catalytic subunit; NEDD8-activating enzyme E1C; Nedd8-activating enzyme hUba3; UBA3; UBA3, ubiquitin-activating enzyme E1 homolog; UBE1C; Ubiquitin-activating enzyme 3; ubiquitin-activating enzyme E1C (homologous to yeast UBA3); Ubiquitin-activating enzyme E1C; ubiquitin-like modifier activating enzyme 3; Ubiquitin-like modifier-activating enzyme 3; yeast)
Molecular Weight
Mass (kDA):
51.852 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 3p14.1 |
| Sequence: | 3; NC_000003.12 (69054730..69080373, complement) |
Tissue Specificity
Ubiquitously expressed.
Diseases
- Malignant Neoplasms
- Malignant Neoplasm Of Breast
- Alzheimer's Disease
- Carcinoma
- Mammary Neoplasms
- Myeloproliferative Syndrome, Transient
- Endoreduplication
- Melanoma
- Neurodegenerative Disorders
- Dementia
- Glioma
- Neurofibromatosis 1
Interacting Proteins
- CPNE2
- IMPA2
- RAD23B
- UBE2M
Pathways
- Conjugation
- Cell Cycle
- Cell Proliferation
- Proteolysis
- S Phase
- Cell Growth
- Rna Interference
- Regulation Of Cell Cycle
- Mitosis
- Membrane Fusion
- Cell Cycle Arrest
- Cell Division
PTMs
- Ubiquitination
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/UBA3
bosterbio.com/bosterbio-gene-info-cards/UBA3
References
PMID: 10207026 by Gong L., et al. Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway.
PMID: 9694792 by Osaka F., et al. A new NEDD8-ligating system for cullin-4A.