UBE2M Antibodies

AND UBE2M ELISA kits, UBE2M Proteins

Many biological assays use antibodies to detect UBE2M, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with UBE2M in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop UBE2M antibodies...

We validate the specificity of these antibodies to UBE2M by testing them on tissues known to express UBE2M positively and negatively. Browse below to find the UBE2M antibody that suites your experiment. We have 3 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing an IHC experiment for UBE2M? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.

Open the UBE2M IHC Guide

UBE2M Infographic by Boster Bio

All UBE2M Products

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Product

Figures

Specificity

Applications

Price

Anti-UBC12/UBE2M Antibody Picoband®

Western blot analysis of UBC12/UBE2M using anti-UBC12/UBE2M antibody (A07478-1)

IHC analysis of UBC12/UBE2M using anti-UBC12/UBE2M antibody (A07478-1)

Human, Mouse, Rat

ELISA, Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # A07478-1
100 μg/vial

UBE2M (NM_003969) Human Over-expression Lysate

Human

$628
Cat # LS009040
20 µg, 100 µg

Human UBE2M/Ubc12 Recombinant Protein

SDS-PAGE

$250
Cat # PROTP61081
Starting from 20ug

UBE2M Overview

Facts about NEDD8-conjugating enzyme Ubc12.

UBE2M 3D structure. Source: alphafold

NEDD8-conjugating enzyme Ubc12 (UBE2M)

Accepts the ubiquitin-like protein NEDD8 in the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4.

Involved in cell proliferation. .

Gene Information

Human
Gene Name:	UBE2M
Uniprot:	P61081
Entrez:	9040

Family

Belongs to:

ubiquitin-conjugating enzyme family

Alternative Names

EC 6.3.2; EC 6.3.2.-; hUbc12; NEDD8 carrier protein; NEDD8 protein ligase; UBC12; UBC12NEDD8-conjugating enzyme Ubc12; UbcH12; UBC-RS2; UBE2M; ubiquitin carrier protein M; Ubiquitin-conjugating enzyme E2 M; ubiquitin-conjugating enzyme E2M (homologous to yeast UBC12); ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast); ubiquitin-protein ligase M; yeast UBC12 homolog

Molecular Weight

Mass (kDA):
20.9 kDA

Genomic Context


Human

Location:	19q13.43
Sequence:	19; NC_000019.10 (58555712..58558611, complement)

Diseases

Malignant Neoplasms
Carcinoma
Bladder Neoplasm
Carcinoma, Transitional Cell
Cancer Patients And Suicide And Depression
Malignant Squamous Cell Neoplasm
Squamous Cell Carcinoma Of Esophagus

Malignant Neoplasm Of Urinary Bladder
Esophageal Neoplasms

Interacting Proteins

CUL1
DCUN1D1
NEDD8
RBX1
UBA3

Pathways

Conjugation
Drug Resistance

PTMs

Acetylation

Methylation

Phosphoprotein

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/UBE2M

References

PMID: 9694792 by Osaka F., et al. A new NEDD8-ligating system for cullin-4A.

PMID: 10207026 by Gong L., et al. Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway.