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- Table of Contents
UCHL1 · Western blot design guide
UCHL1 Western Blot Planning Guide
Plan an UCHL1 Western blot around the catalog-observed 24.8 kDa band, image-backed M01018-6 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
UCHL1 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 24.8 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Hippocampus | |
| Negative control | Appendix |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 24.8 kDa |
| ⓘ | Localization | Cytoplasm / Endoplasmic reticulum membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated UCHL1 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Caudate |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, short transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | M01018-6 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected UCHL1 Western Blot Band Size?
Use the product-observed 24.8 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 24.8 kDa | Matches the authoritative product WB observation. |
| 24.8 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected UCHL1 appearancePlan around 24.8 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 24.8 kDa; use this as the primary experimental expectation. |
| Calculated mass | 24.8 kDa from UniProt P09936; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with M01018-6. |
| Situation | Likely cause | Next action |
|---|---|---|
| 24.8 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing UCHL1 band
M01018-6 · UCHL1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for UCHL1 Western blot
🧪Use Hippocampus as the first positive-control candidate and Appendix as the HPA Not detected negative candidate.
Positive control: Hippocampus (High)
Negative control: Appendix (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for UCHL1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Hippocampus | Reported tissue cells | High | Protein (HPA) | HPA → |
| Caudate | Reported tissue cells | High | Protein (HPA) | HPA → |
| Cerebellum | Reported tissue cells | High | Protein (HPA) | HPA → |
| Kidney | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 3
Advanced UCHL1 Western Blot Tips
Deeper troubleshooting and optimisation questions for UCHL1, answered from its protein features.
Which band should guide the blot?
Use 24.8 kDa, the observation attached to the authoritative M01018-6 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 24.8 kDa expectation.
Which positive control should I start with?
Start with Hippocampus, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Appendix as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for UCHL1 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should M01018-6 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
Compare only the supplied target-verified records: PMC6718320, PMC7164950, PMC5584584.
Boster reagents
UCHL1 Western Blot Reagents
Human/Mouse/Rat-reactive UCHL1 Western blot reagents with authoritative product imagery.
Real WB data
Anti-PGP9.5 Antibody Picoband® (monoclonal, 3E4)
Cat # M01018-6
Real WB data
Anti-PGP9.5/UCHL1 Antibody Picoband®
Cat # PB9840
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P09936
- Human Protein Atlas — UCHL1
- M01018-6 product record
- PMC6718320 — UCHL1 regulates muscle fibers and mTORC1 activity in skeletal muscle.
- PMC7164950 — The deubiquitinase UCHL1 regulates cardiac hypertrophy by stabilizing epidermal growth factor receptor.
- PMC5584584 — Ubiquitin C-Terminal Hydrolase L1 regulates myoblast proliferation and differentiation.