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WDR83 / WD repeat domain-containing protein 83 · IHC design guide
Design Immunohistochemistry for WDR83
Plan WDR83 chromogenic IHC in paraffin sections using its reported cytoplasmic tissue staining as a starting point (HPA tissue IHC). The guide covers controls, staining assessment, and the uncertainty caused by low antibody–RNA concordance (HPA tissue IHC).
Evidence assembled Oct 2026 · For research use; verify linked source records and product datasheet before use
WDR83 Immunohistochemistry Experimental Design Guide
Expected localisation, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before staining
| Expected localisation | Cytoplasmic in tissue (HPA tissue IHC); partial nuclear localisation possible (UniProt) | |
| Staining pattern | Widespread cytoplasmic staining (HPA tissue IHC) | |
| Antigen retrieval | Tris-EDTA pH 9.0 HIER, 95–98 °C, 20 min (rule: nuclear antigen) | |
| Positive control | Adrenal gland+4 more · see all | |
| Negative control | Bone marrow+3 more · see all |
Important caveats
Reasons your staining may differ from the expected pattern.
| Fixation | Keep fixation consistent across paraffin sections. (standard IHC practice; not target-specific) | |
| Caveat | Antibody staining has low concordance with RNA data (HPA tissue IHC) | |
| Regulation | Expression regulation unreported (UniProt) | |
| Isoform / epitope | No isoforms or processing annotated; epitope impact unknown (UniProt) |
Section 1
Recommended WDR83 IHC & IF Protocols
The catalog antibody’s IHC-P protocol is paired with a published chromogenic WDR83 protocol for mouse brain (PMC12816048).
| Sample | Tissue sections; selected-image fixative not specified (standard IHC workflow) |
| Fixation | Image fixative and duration unreported (datasheet PA1053); verify before use. |
| Sectioning | 4–5 µm sections on charged slides (standard) |
| Deparaffinisation | Xylene, graded ethanol series to water (standard) |
| Antigen retrieval | Heat-induced epitope retrieval in Tris-EDTA buffer, pH 9.0, 20 min at 95–98 °C (standard rule: nuclear antigen) |
| Peroxidase block | 3% H2O2, 10 min, room temperature (standard) |
| Blocking | 10% normal serum of the secondary host, 30 min, room temperature (standard) |
| Primary antibody | Rabbit anti-WDR83, 0.5-1μg/ml (datasheet PA1053) |
| Primary incubation | Overnight at 4 °C (standard) |
| Detection | HRP-polymer secondary, DAB chromogen 5–10 min (standard) |
| Counterstain | Hematoxylin, blue, dehydrate and mount (standard) |
| Expected result | WDR83-positive staining in glandular cells of adrenal gland (HPA tissue IHC: Medium). HPA tissue profile: Ubiquitous cytoplasmic expression. No signal in the no-primary control. |
💡Decision noteStart with Tris-EDTA HIER at pH 9.0, 95–98 °C for 20 min (page antigen-retrieval setting); the published protocol used 70 °C retrieval (PMC12816048).
Section 2
What Is the Expected WDR83 Staining Pattern?
WDR83 is predominantly cytoplasmic and partly nuclear, with lysosomal localization; it has no transmembrane segment (UniProt Q9BRX9 localization and topology). In paraffin sections, look for cytoplasmic staining in the cell types where HPA reports medium signal, such as kidney tubular cells and liver hepatocytes (HPA tissue IHC). Treat the pattern as provisional: HPA rates tissue IHC reliability Uncertain because antibody staining and RNA expression have low consistency (HPA tissue IHC).
| Cytoplasmic staining in kidney tubular cells or liver hepatocytes, with possible nuclear signal. | This is compatible with the reported medium IHC staining in those cells (HPA tissue IHC) and predominantly cytoplasmic, partly nuclear localization (UniProt Q9BRX9). Judge whether the cytoplasmic signal is distinguishable from background on the same section; this pattern alone cannot establish specificity because tissue IHC reliability is Uncertain (HPA tissue IHC). |
| Signal appears only at cell borders, in extracellular material, or as a uniform surface outline. | That distribution does not match the reported intracellular localization (UniProt Q9BRX9; HPA subcellular ICC-IF). WDR83 has no transmembrane segment (UniProt Q9BRX9 topology). Check the counterstain and tissue structure before assigning a compartment; a boundary pattern warrants investigation as possible artifact rather than a WDR83-positive call. |
| Strong staining appears in bone marrow hematopoietic cells while expected positive cells are weak. | HPA reports WDR83 as not detected in bone marrow hematopoietic cells (HPA tissue IHC). Consider cross-reactivity or endogenous chromogenic detection activity (general IHC practice). Because HPA rates its tissue IHC evidence Uncertain, this comparison flags a result for control checks; it does not prove the stained cells lack WDR83. |
| Brown signal spreads across the section without clear cell boundaries or compartment contrast. | Treat this as diffuse background until staining can be assigned to cells and intracellular compartments (general IHC interpretation). It cannot be matched confidently to the cytoplasmic tissue pattern (HPA tissue IHC) or predominant cytoplasmic localization (UniProt Q9BRX9). Review a no-primary control and the detection and blocking steps (general IHC practice). |
| No staining is visible in kidney tubular cells or liver hepatocytes. | HPA reports medium IHC staining in these cell types (HPA tissue IHC), so an entirely blank result calls for a technical check. Confirm tissue identity and examine a positive-control section processed in the same run (general IHC practice). An absent result alone cannot resolve biological absence versus assay failure, especially with Uncertain HPA tissue IHC reliability. |
💡Expected WDR83 appearanceCall a section provisionally positive when identifiable kidney tubular cells or liver hepatocytes show discernible, approximately medium cytoplasmic staining, with possible partial nuclear staining (HPA tissue IHC; UniProt Q9BRX9 localization); isolated extracellular or cell-border color is a suspect positive because the reported locations are intracellular (UniProt Q9BRX9; HPA subcellular ICC-IF).
| Tissue evidence and confidence (HPA tissue IHC) | HPA describes a ubiquitous cytoplasmic profile and low tissue RNA specificity, yet rates tissue IHC reliability Uncertain because staining and RNA have low consistency (HPA tissue IHC). Use reported medium-staining cells as practical comparison sites, not proof that every section or antibody run must stain at the same intensity. |
| Compartment evidence across assays (UniProt Q9BRX9; HPA subcellular ICC-IF) | UniProt calls WDR83 predominantly cytoplasmic and partly nuclear, with lysosomal localization (UniProt Q9BRX9). HPA ICC-IF instead lists nucleoplasm and vesicles as main approved locations, with cytosol among additional locations (HPA subcellular ICC-IF). Read those as assay-specific observations; the ICC-IF pattern does not set an IHC nuclear-intensity threshold. |
| Comparator cell types (HPA tissue IHC) | HPA reports medium staining in bronchial respiratory epithelial cells, cerebellar Purkinje cells, colon and gallbladder glandular cells, adrenal glandular cells, cardiomyocytes, kidney tubular cells, and hepatocytes (HPA tissue IHC). It reports no detection in bone marrow hematopoietic cells, ovarian stroma cells, salivary gland glandular cells, and vaginal squamous epithelial cells (HPA tissue IHC). Compare named cells, not whole-organ averages. |
| Topology and processing (UniProt Q9BRX9) | The annotated protein is a single 1–315 chain without a signal peptide, propeptide, or transmembrane segment; no glycosylation sites or modified residues are annotated (UniProt Q9BRX9). This supports checking an intracellular pattern. These annotations provide no basis for predicting epitope exposure after fixation or choosing an antigen-retrieval condition. |
| Situation | Likely cause | Next action |
|---|---|---|
| No signal in a reported medium-staining cell type (HPA tissue IHC). | Possible run failure, weak detection, or tissue mismatch (general IHC practice); HPA reliability is Uncertain (HPA tissue IHC). | Verify cell identity, inspect a same-run positive control, and review antibody dilution, retrieval, and detection settings against the assay instructions (general IHC practice). Do not infer a WDR83-specific fixation effect. |
| Diffuse chromogen obscures cytoplasmic detail. | Background from detection or incomplete blocking may prevent compartment scoring (general IHC practice). | Examine a no-primary control; review blocking, wash, and chromogen development steps (general IHC practice). Score WDR83 only where intracellular signal remains distinguishable from background. |
| Stain appears exclusively extracellular or at cell boundaries. | The pattern conflicts with reported intracellular locations and absence of a transmembrane segment (UniProt Q9BRX9; HPA subcellular ICC-IF). | Recheck tissue structure and counterstain, then compare with a no-primary control (general IHC practice). Withhold a positive call until the apparent compartment is resolved. |
| Bone marrow hematopoietic cells stain strongly. | HPA reports them as not detected (HPA tissue IHC); cross-reactivity or endogenous detection activity is possible (general IHC practice). | Compare a no-primary control and reported medium-staining cells processed in the same run (general IHC practice; HPA tissue IHC). Treat the discrepancy as unresolved because HPA tissue IHC reliability is Uncertain. |
| Sections vary in the number of stained cells. | HPA levels refer to named cell types, and tissue IHC reliability is Uncertain (HPA tissue IHC); section composition can also vary (general histology practice). | Identify and score the relevant cells within each section before comparing runs (general IHC practice). Record intensity and compartment separately instead of treating every unstained cell as assay failure. |
| IF/ICC Q&A: where should WDR83 appear? | HPA ICC-IF lists nucleoplasm and vesicles as approved main locations; cytosol, centriolar satellites, and basal body are additional approved locations (HPA subcellular ICC-IF). | Interpret IF/ICC localization using its own guide and controls (general IF practice). Do not transfer IF/ICC compartment prominence into an IHC staining-intensity rule (HPA subcellular ICC-IF; HPA tissue IHC). |
Experiment evidence
IHC & IF images showing WDR83 staining
PA1053 · IHCAnti-Morg1 antibody, PA1053, IHC(P) IHC(P): Rat Lung Tissue
HPA · Cerebral cortexHuman Protein Atlas IHC, Cerebral cortex. Source: https://www.proteinatlas.org/ENSG00000123154-WDR83/tissue/cerebral+cortex#imid_11332994
HPA · ColonHuman Protein Atlas IHC, Colon. Source: https://www.proteinatlas.org/ENSG00000123154-WDR83/tissue/colon#imid_11332933
HPA · LiverHuman Protein Atlas IHC, Liver. Source: https://www.proteinatlas.org/ENSG00000123154-WDR83/tissue/liver#imid_11332926
HPA · KidneyHuman Protein Atlas IHC, Kidney. Source: https://www.proteinatlas.org/ENSG00000123154-WDR83/tissue/kidney#imid_11332935
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody HPA042629; green = WDR83, blue = nucleus, red = microtubules.
HPA · ICC-IF A-431Human Protein Atlas immunofluorescence, A-431, antibody HPA042629; green = WDR83, blue = nucleus, red = microtubules.
← swipe / scroll for more images →
Real IHC / IF data behind the staining-pattern expectations in this section.
Sample controls for WDR83 IHC & IF
🧪Run colon first: its glandular cells should stain at the HPA Medium level (HPA: colon glandular cells, Medium). Use salivary gland glandular cells as the negative tissue (HPA: salivary gland glandular cells, Not detected); on the colon slide, use adjacent unstained cells as background comparators without assuming they are WDR83-negative.
Positive control tissue: Adrenal gland (Glandular cells, HPA Medium)
Negative control tissue: Bone marrow (HPA Not detected)
ICC-IF cell lines (HPA subcellular resource): HPA ICC-IF images show WDR83 in A-431, U-251MG, U2OS, ASC52telo, RPTEC/TERT1, hTERT-RPE1 (serum starved), with annotated localisation: Nucleoplasm (approved), Vesicles (approved) (HPA subcellular).
Technical controls: Include a no-primary, secondary-only slide and a host- and isotype-matched control IgG for a monoclonal primary, or host-matched nonimmune IgG for a polyclonal primary; use WDR83 knockout material or peptide competition, if available, to assess specificity. Block endogenous peroxidase for chromogenic detection in colon, and check for endogenous biotin if using a biotin-based detection system (HPA: colon glandular cells, Medium).
⚠️Feasibility: A target-specific fixation window and antigen-retrieval dependency are unreported in the supplied evidence; the PA1053 rat-lung IHC(P) caption does not state its fixative (PA1053 caption). The evidence does not establish whether frozen sections or IF/ICC would be easier, though HPA provides ICC-IF images and reports nucleoplasm and vesicle localization (HPA: subcellular). In colon, compare glandular-cell staining with the no-primary slide to distinguish signal from luminal debris or peroxidase background (HPA: colon glandular cells, Medium).
HPA tissue IHC evidence for WDR83
Comprehensive Human Protein Atlas IHC scoring per tissue (reliability: Uncertain — Low consistency between antibody staining and RNA expression data. Pending external verification.). Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adrenal gland | Glandular cells | Medium | Protein (IHC) | HPA → |
| Bronchus | Respiratory epithelial cells | Medium | Protein (IHC) | HPA → |
| Cerebellum | Purkinje cells | Medium | Protein (IHC) | HPA → |
| Colon | Glandular cells | Medium | Protein (IHC) | HPA → |
| Gallbladder | Glandular cells | Medium | Protein (IHC) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Bone marrow | Hematopoietic cells | Not detected | Protein (IHC) | HPA → |
| Ovary | Ovarian stroma cells | Not detected | Protein (IHC) | HPA → |
| Salivary gland | Glandular cells | Not detected | Protein (IHC) | HPA → |
| Vagina | Squamous epithelial cells | Not detected | Protein (IHC) | HPA → |
Section 3
Advanced WDR83 IHC Tips
Troubleshoot WDR83 staining in paraffin sections by checking retrieval, compartment pattern, controls, and scoring before interpreting chromogenic signal.
Which retrieval conditions should I use when WDR83 staining is weak?
Use Tris-EDTA at pH 9.0 for heat-induced retrieval at 95–98 °C for 20 min (page retrieval setting). Keep section thickness, heating vessel, cooling period, and antibody incubation consistent while comparing runs, because retrieval changes can alter both signal and tissue integrity (standard IHC practice). If staining remains weak, test a shorter or longer heating interval on matched sections before trying another buffer; record any tissue lifting or increased background (standard IHC practice). A rat lung paraffin-section image exists for PA1053, but its caption supplies no retrieval conditions, so it cannot establish which treatment produced that image (PA1053 caption).
Could fixation explain uneven WDR83 staining across my paraffin sections?
WDR83-specific fixation sensitivity is unknown from the supplied evidence; the PA1053 rat lung paraffin-section caption does not state a fixative (PA1053 caption). Record fixative, time before immersion, fixation duration, processing schedule, and section age for every specimen before comparing staining intensity (standard IHC practice). For a controlled repeat, fix matched samples under the same documented conditions and process them together, then apply the page retrieval setting of pH 9.0 at 95–98 °C for 20 min (page retrieval setting; standard IHC practice). Do not assign a fixation effect to WDR83 from its tissue distribution or predicted protein features alone (HPA tissue IHC; UniProt Q9BRX9).
How should I evaluate nuclear and punctate WDR83 staining?
Score cytoplasmic and nuclear staining separately: UniProt describes WDR83 as predominantly cytoplasmic and partially nuclear, with lysosomal localisation also reported (UniProt Q9BRX9). The subcellular atlas instead reports nucleoplasm and vesicles as main locations, with cytosol among additional locations; these observations support checking more than one compartment (HPA subcellular). In chromogenic sections, require recognisable cell boundaries and a counterstained nucleus before calling a signal nuclear or cytoplasmic (standard IHC practice). For puncta, compare serial sections, no-primary controls, and tissue morphology; brown granules alone do not identify lysosomes or establish WDR83 specificity (standard IHC practice).
What should I check if the antibody gives an unexpected staining pattern?
First check the catalog antibody’s immunogen or epitope documentation against the full 315-amino-acid WDR83 sequence; no epitope location is supplied here (UniProt Q9BRX9; supplied antibody evidence). The provided record lists no annotated isoforms, signal peptide, propeptide, glycosylation sites, or transmembrane segment, so those features do not explain compartment-specific staining in these sections (UniProt Q9BRX9). Compare staining after the specified pH 9.0, 20 min retrieval with a matched control and document any shift in nuclear versus cytoplasmic signal (page retrieval setting; standard IHC practice). If available, use an independently validated antibody or WDR83-depleted material to test specificity before interpreting an unusual pattern (standard IHC practice).
How can IF help assess an ambiguous WDR83 IHC pattern?
Use IF as a separate localisation check and multiplex WDR83 with a marker identifying the cell population being scored in the chromogenic section; choose that marker for the specimen under study (standard IF practice). Select fluorophores after inspecting unstained tissue autofluorescence, and favor a spectrally separated, brighter channel for the weaker signal (standard IF practice). WDR83 has no transmembrane segment and is reported in intracellular compartments, so permeabilise adequately for intracellular epitope access while checking that punctate structure is preserved; the antibody’s exact epitope is unspecified (UniProt Q9BRX9; standard IF practice). Compare compartment patterns cautiously: the subcellular atlas reports nucleoplasm and vesicles, but its IF observations do not establish the performance of PA1053 in paraffin sections (HPA subcellular; PA1053 caption).
What should I change when brown staining obscures WDR83 signal?
Inspect a no-primary section first; persistent brown deposits there point toward detection-system or tissue background rather than primary-antibody binding (standard IHC practice). For peroxidase-based chromogenic IHC, include an endogenous-peroxidase block and control DAB development time consistently across matched sections (standard IHC practice). If background appears only with primary antibody, titrate its working concentration, strengthen blocking or washing as appropriate, and compare with a matched control section (standard IHC practice). Check section edges, folds, pigment, and necrotic areas before scoring; the HPA tissue profile describes widespread cytoplasmic staining but assigns WDR83 tissue IHC an Uncertain reliability rating (standard IHC practice; HPA tissue IHC).
How should I quantify WDR83 staining across specimens? ⚠ ANSWER MARKED FOR VERIFICATION
Define the cell population and compartment before scoring, then report either the percentage of positive cells with intensity categories or an H-score calculated as the summed percentage in each 0–3 intensity category multiplied by that category (standard IHC practice). For punctate staining, density per mm² of viable tissue can supplement cell-level scoring when segmentation is reproducible (standard image-analysis practice). Normalise to the number of eligible cells or viable tissue area, and keep staining batch, imaging settings, and thresholds consistent (standard IHC practice). Report nuclear and cytoplasmic results separately because both are plausible WDR83 locations, and flag the atlas’s Uncertain tissue-IHC reliability when interpreting differences (UniProt Q9BRX9; HPA tissue IHC).
When is a positive WDR83 IHC result convincing?
A convincing result has reproducible signal in intact cells, a plausible cytoplasmic or partial nuclear distribution, and low signal in the relevant negative controls (UniProt Q9BRX9; standard IHC practice). Consider cell identity: the atlas reports medium staining in bronchial respiratory epithelial cells and kidney tubular cells, while bone marrow hematopoietic cells are listed as not detected, but its tissue-IHC reliability is Uncertain (HPA tissue IHC). Reconsider staining confined to section edges, folds, necrosis, extracellular material, or a no-primary control; endogenous enzyme activity can also create chromogenic signal (standard IHC practice). Treat an isolated compartment or cell-type mismatch as a prompt for independent validation, not proof of altered WDR83 biology (standard IHC practice).
Boster reagents
Best WDR83 / WD repeat domain-containing protein 83 IHC Antibodies
PA1053 provides IHC data from rat lung paraffin sections (PA1053 image caption) and lists human, mouse and rat reactivity (PA1053 catalog). No IF data are supplied (PA1053 catalog).
Real IHC data
Anti-Morg1/WDR83 Antibody ®
Cat # PA1053
PA1053 is listed for IHC and WB, with human, mouse and rat reactivity (PA1053 catalog). Its IHC(P) image shows rat lung tissue; no IF image is supplied (PA1053 image caption; PA1053 catalog).
Which to pick: Choose PA1053 for tissue IHC in paraffin sections: its own image shows rat lung IHC(P), and its listed IHC concentration is 0.5–1 μg/ml (PA1053 image caption; PA1053 datasheet). Its fixative is unreported (PA1053 image caption). For cross-species work, PA1053 lists human, mouse and rat reactivity, but the supplied IHC image documents rat tissue only; no IF/ICC option is documented because IF/ICC is absent from its application list and no IF image is supplied (PA1053 catalog; PA1053 image caption).
Each figure is that product's own IHC / IF validation image from its datasheet.
References
- UniProt Consortium. UniProt entry Q9BRX9 (WDR83_HUMAN, WD repeat domain-containing protein 83).
- Human Protein Atlas. WDR83 tissue IHC expression (reliability: Uncertain).
- Human Protein Atlas. WDR83 subcellular location (ICC-IF): Mainly localized to the nucleoplasm and vesicles. In addition localized to the cytosol, centriolar satellites and basal body..
- Human Protein Atlas. WDR83 antibody validation summary (2 antibodies).
- The p.L218P variant in WDR83 disrupts neuronal development, leading to neurodevelopmental disorder. Scientific reports 2025 — PMC12816048.
- PubMed PMID:14702039 — UniProt-cited evidence.
- PubMed PMID:15489334 — UniProt-cited evidence.
- PubMed PMID:11991638 — UniProt-cited evidence.
Immunohistochemistry protocol sheet for WDR83 (WD repeat domain-containing protein 83). Expected localisation Cytoplasmic in tissue (HPA tissue IHC); partial nuclear localisation possible (UniProt). Antigen retrieval: Tris-EDTA pH 9.0 HIER, 95–98 °C, 20 min (rule: nuclear antigen). Validated IHC antibody: PA1053 at 0.5-1μg/ml (the product's own IHC validation image is shown). Controls (HPA): positive Adrenal gland (Glandular cells); negative Bone marrow. IHC workflow; specimen preparation unspecified. Protocol steps: 1. Sample: Tissue sections; selected-image fixative not specified (standard IHC workflow); 2. Fixation: Image fixative and duration unreported (datasheet PA1053); verify before use.; 3. Sectioning: 4–5 µm sections on charged slides (standard); 4. Deparaffinisation: Xylene, graded ethanol series to water (standard); 5. Antigen retrieval: Heat-induced epitope retrieval in Tris-EDTA buffer, pH 9.0 , 20 min at 95–98 °C…; 6. Peroxidase block: 3% H2O2, 10 min , room temperature (standard); 7. Blocking: 10% normal serum of the secondary host, 30 min , room temperature (standard); 8. Primary antibody: Rabbit anti-WDR83, 0.5-1μg/ml (datasheet PA1053); 9. Primary incubation: Overnight at 4 °C (standard). Watch for: Fixation: Keep fixation consistent across paraffin sections. (standard IHC practice; not target-specific); Caveat: Antibody staining has low concordance with RNA data (HPA tissue IHC); Isoform / epitope: No isoforms or processing annotated; epitope impact unknown (UniProt); Regulation: Expression regulation unreported (UniProt). Evidence: UniProt Q9BRX9, HPA tissue IHC · Uncertain, HPA ICC-IF · Mainly localized to the nucleoplasm and vesicles. In addition…, Datasheet PA1053, PMC12816048. QR code links to https://www.bosterbio.com/bosterbio-gene-info-cards/WDR83/immunohistochemistry-ihc-guide.