Calsenilin/KCNIP3 Colorimetric Cell-Based ELISA Kit

The Calsenilin/KCNIP3 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Calsenilin/KCNIP3 protein expression profile in cells. The kit can be used for measuring the relative amounts of Calsenilin/KCNIP3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Calsenilin/KCNIP3.

Product Info Summary

SKU: EKC1076
Size: 1 kit, containing 1 plates and all necessary reagents
Reactive Species: Human, Mouse
Application: ELISA

Product Name

Calsenilin/KCNIP3 Colorimetric Cell-Based ELISA Kit

See all Calsenilin products

SKU/Catalog Number

EKC1076

Size

1 kit, containing 1 plates and all necessary reagents

*Question: How many samples can I assay/run in this kit?

Description

The Calsenilin/KCNIP3 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Calsenilin/KCNIP3 protein expression profile in cells. The kit can be used for measuring the relative amounts of Calsenilin/KCNIP3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Calsenilin/KCNIP3.

Storage & Handling

4°C/6 Months

Cite This Product

Calsenilin/KCNIP3 Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1076)

Clonality of Antibodies

See Datasheet for details

Sensitivity

>5000 cells

Assay Range

> 5000 Cells

Reactive Species

EKC1076 is reactive to KCNIP3 in Human, Mouse samples

Application Guarantee

EKC1076 is guaranteed for ELISA in Human, Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-Calsenilin Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Gene/Protein Information For KCNIP3 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9Y2W7

Gene ID

30818

Gene Name

KCNIP3

Full Name

Calsenilin

Weight

29.231kDa

Superfamily

recoverin family

Alternative Names

calsenilin; calsenilin, presenilin-binding protein, EF hand transcription factor; CSEN MGC18289; DREAM; DREAMA-type potassium channel modulatory protein 3; KCHIP3 DRE-antagonist modulator; KChIP3; Kv channel interacting protein 3, calsenilin; Kv channel-interacting protein 3; potassium channel interacting protein 3 KCNIP3 CSEN, DREAM, KCHIP3 potassium voltage-gated channel interacting protein 3 calsenilin|A-type potassium channel modulatory protein 3|DRE-antagonist modulator|Kv channel interacting protein 3, calsenilin|calsenilin, presenilin-binding protein, EF hand transcription factor|kv channel-interacting protein 3|potassium channel interacting protein 3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on KCNIP3, check out the KCNIP3 Infographic

KCNIP3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for KCNIP3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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