Caspase 8 (CASP8) (NM_001228) Human Over-expression Lysate

Lysate of Caspase-8 over-expression cell lines, WB control

Transient overexpression lysate of caspase 8, apoptosis-related cysteine peptidase (CASP8), transcript variant A

Product Info Summary

SKU: LS000841
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of caspase 8, apoptosis-related cysteine peptidase (CASP8), transcript variant A

Product Name

Caspase 8 (CASP8) (NM_001228) Human Over-expression Lysate

See all Caspase-8 products

SKU/Catalog Number

LS000841

Size

100 µg

Description

Transient overexpression lysate of caspase 8, apoptosis-related cysteine peptidase (CASP8), transcript variant A

Storage & Handling

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Cite This Product

Caspase 8 (CASP8) (NM_001228) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS000841)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for CASP8 (Source: Uniprot.org, NCBI)

Gene Name

CASP8

Full Name

Caspase-8

Weight

55.391kDa

Superfamily

peptidase C14A family

Alternative Names

AIS; androgen receptor; CASP8; Caspase8; Caspase-8; DHTRTFM; Dihydrotestosterone receptorHYSP1; HUMARA; Mch5; NR3C4KD; Nuclear receptor subfamily 3 group C member 4; SMAX1SBMA; spinal and bulbar muscular atrophy CASP8 ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5 caspase 8 caspase-8|FADD-homologous ICE/CED-3-like protease|FADD-like ICE|ICE-like apoptotic protease 5|MACH-alpha-1/2/3 protein|MACH-beta-1/2/3/4 protein|MORT1-associated ced-3 homolog|apoptotic cysteine protease|apoptotic protease Mch-5|caspase 8, apoptosis-related cysteine peptidase|caspase 8, apoptosis-related cysteine protease

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on CASP8, check out the CASP8 Infographic

CASP8 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CASP8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Loading publications

No publications found

Do you have publications using this product? Share with us and receive a reward. Contact us for more information.

Publications not available. Please try again later.

Product has been cited in publications

No results found matching your query

  • Published: --- Journal: --- Application: --- Impact Factor: --- Species: --- PMID: None

Have you used Caspase 8 (CASP8) (NM_001228) Human Over-expression Lysate?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Caspase 8 (CASP8) (NM_001228) Human Over-expression Lysate

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for Caspase 8 (CASP8) (NM_001228) Human Over-expression Lysate

$960
Backordered.

Lead time for this item is typically 2 weeks

Get A Quote
In stock
Order Product
LS000841
$960.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.