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Product Info Summary
|Description:||Transient overexpression lysate of selectin P (granule membrane protein 140kDa, antigen CD62) (SELP)|
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CD62P (SELP) (NM_003005) Human Over-expression Lysate
Transient overexpression lysate of selectin P (granule membrane protein 140kDa, antigen CD62) (SELP)
Storage & Handling
Cite This Product
CD62P (SELP) (NM_003005) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006403)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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CD62P (SELP) Human Over-expression Lysates NM_003005.
Western blot validation of overexpression lysate (LS006403) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SELP (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SELP (Source: Uniprot.org, NCBI)
CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; P-Selectin; selectin P (granule membrane protein 140kDa, antigen CD62); SELP SELP CD62, CD62P, GMP140, GRMP, LECAM3, PADGEM, PSEL selectin P P-selectin|CD62 antigen-like family member P|GMP-140|granule membrane protein 140|granule membrane protein 140kDa|granulocyte membrane protein|leukocyte-endothelial cell adhesion molecule 3|platelet activation dependent granule-external membrane protein|platelet alpha-granule membrane protein|selectin P (granule membrane protein 140kDa, antigen CD62)*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on SELP, check out the SELP Infographic
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In this infographic, you will see the following information for SELP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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