CRMP2 (Phospho-Thr509) Colorimetric Cell-Based ELISA Kit

CRMP2 ELISA kit, phospho-specific, cell-based

The CRMP2 (Phospho-Thr509) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CRMP2 (Phospho-Thr509) protein expression profile in cells. The kit can be used for measuring the relative amounts of CRMP2 (Phospho-Thr509) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on CRMP2 (Phospho-Thr509).

Product Info Summary

SKU: EKC2611
Size: 1 kit, containing two 96-well plates and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA

Product Name

CRMP2 (Phospho-Thr509) Colorimetric Cell-Based ELISA Kit

See all CRMP2 products

SKU/Catalog Number

EKC2611

Size

1 kit, containing two 96-well plates and all necessary reagents

Description

The CRMP2 (Phospho-Thr509) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CRMP2 (Phospho-Thr509) protein expression profile in cells. The kit can be used for measuring the relative amounts of CRMP2 (Phospho-Thr509) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on CRMP2 (Phospho-Thr509).

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

CRMP2 (Phospho-Thr509) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2611)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC2611 is reactive to DPYSL2 in Human, Mouse, Rat samples

Application Guarantee

EKC2611 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-CRMP2 Phospho-Thr509 Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-CRMP2 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For DPYSL2 (Source: Uniprot.Org, NCBI)

Gene Name

DPYSL2

Full Name

Dihydropyrimidinase-related protein 2

Weight

62294 MW

Superfamily

metallo-dependent hydrolases superfamily

Alternative Names

Dihydropyrimidinase-related protein 2;DRP-2;Collapsin response mediator protein 2;CRMP-2;N2A3;Unc-33-like phosphoprotein 2;ULIP-2;DPYSL2;CRMP2, ULIP2; DPYSL2 CRMP-2, CRMP2, DHPRP2, DRP-2, DRP2, N2A3, ULIP-2, ULIP2 dihydropyrimidinase like 2 dihydropyrimidinase-related protein 2|collapsin response mediator protein hCRMP-2|unc-33-like phosphoprotein 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on DPYSL2, check out the DPYSL2 Infographic

DPYSL2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for DPYSL2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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