DBF4B (NM_145663) Human Over-expression Lysate

Lysate of DBF4B over-expression cell lines, WB control

Transient overexpression lysate of DBF4 homolog B (S. cerevisiae) (DBF4B), transcript variant 1

Product Info Summary

SKU: LS080174
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of DBF4 homolog B (S. cerevisiae) (DBF4B), transcript variant 1

Product Name

DBF4B (NM_145663) Human Over-expression Lysate

See all DBF4B products

SKU/Catalog Number

LS080174

Size

100 µg

Description

Transient overexpression lysate of DBF4 homolog B (S. cerevisiae) (DBF4B), transcript variant 1

Storage & Handling

Cite This Product

DBF4B (NM_145663) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS080174)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for DBF4B (Source: Uniprot.org, NCBI)

Gene Name

DBF4B

Full Name

Protein DBF4 homolog B

Weight

67.243kDa

Alternative Names

Activator of S phase kinase-like protein 1; activator of S-phase kinase-like protein 1; ASKL1CHIFB; ASK-like protein 1; chifb; Chiffon homolog B; DBF4 homolog B (S. cerevisiae); Dbf4-related factor 1; DRF1MGC15009; FLJ13087; protein DBF4 homolog B; ZDBF1B; zinc finger, DBF-type containing 1B DBF4B ASKL1, CHIFB, DRF1, ZDBF1B DBF4 zinc finger B protein DBF4 homolog B|ASK-like protein 1|DBF4 homolog B|Dbf4-related factor 1|activator of S-phase kinase-like protein 1|chiffon homolog B|zinc finger, DBF-type containing 1B

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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