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Product Info Summary
|Description:||Transient overexpression lysate of DBF4 homolog B (S. cerevisiae) (DBF4B), transcript variant 1|
DBF4B (NM_145663) Human Over-expression Lysate
Transient overexpression lysate of DBF4 homolog B (S. cerevisiae) (DBF4B), transcript variant 1
Storage & Handling
Cite This Product
DBF4B (NM_145663) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS080174)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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DBF4B Human Over-expression Lysates NM_145663.
Western blot validation of overexpression lysate (LS080174) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with DBF4B (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for DBF4B (Source: Uniprot.org, NCBI)
Protein DBF4 homolog B
Activator of S phase kinase-like protein 1; activator of S-phase kinase-like protein 1; ASKL1CHIFB; ASK-like protein 1; chifb; Chiffon homolog B; DBF4 homolog B (S. cerevisiae); Dbf4-related factor 1; DRF1MGC15009; FLJ13087; protein DBF4 homolog B; ZDBF1B; zinc finger, DBF-type containing 1B DBF4B ASKL1, CHIFB, DRF1, ZDBF1B DBF4 zinc finger B protein DBF4 homolog B|ASK-like protein 1|DBF4 homolog B|Dbf4-related factor 1|activator of S-phase kinase-like protein 1|chiffon homolog B|zinc finger, DBF-type containing 1B*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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