DGKI Colorimetric Cell-Based ELISA

The DGKI Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor DGKI protein expression profile in cells. The kit can be used for measuring the relative amounts of DGKI in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on DGKI.

Product Info Summary

SKU: EKC1655
Size: 1 kit, containing 1 plates and all necessary reagents
Reactive Species: Human, Rat
Application: ELISA

Product Name

DGKI Colorimetric Cell-Based ELISA

See all DGK-iota products

SKU/Catalog Number

EKC1655

Size

1 kit, containing 1 plates and all necessary reagents

*Question: How many samples can I assay/run in this kit?

Description

The DGKI Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor DGKI protein expression profile in cells. The kit can be used for measuring the relative amounts of DGKI in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on DGKI.

Storage & Handling

4°C/6 Months

Cite This Product

DGKI Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1655)

Clonality of Antibodies

See Datasheet for details

Sensitivity

>5000 cells

Assay Range

> 5000 Cells

Reactive Species

EKC1655 is reactive to DGKI in Human, Rat samples

Application Guarantee

EKC1655 is guaranteed for ELISA in Human, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-DGK-iota Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For DGKI (Source: Uniprot.Org, NCBI)

Uniprot ID

O75912

Gene ID

9162

Gene Name

DGKI

Full Name

Diacylglycerol kinase iota

Weight

116.997kDa

Superfamily

eukaryotic diacylglycerol kinase family

Alternative Names

DAG kinase iota; DGKI; DGKiota; DGK-iota; diacylglycerol kinase iota; diacylglycerol kinase, iota; Diglyceride kinase iota; EC 2.7.1.107 DGKI DGK-IOTA diacylglycerol kinase iota diacylglycerol kinase iota|DAG kinase iota|diglyceride kinase iota

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on DGKI, check out the DGKI Infographic

DGKI infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for DGKI: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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