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Product Info Summary
|Description:||Transient overexpression lysate of solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 (SLC1A1)|
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EAAT3 (SLC1A1) (NM_004170) Human Over-expression Lysate
Transient overexpression lysate of solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 (SLC1A1)
Storage & Handling
Cite This Product
EAAT3 (SLC1A1) (NM_004170) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006505)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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EAAT3 (SLC1A1) Human Over-expression Lysates NM_004170.
Western blot validation of overexpression lysate (LS006505) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SLC1A1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SLC1A1 (Source: Uniprot.org, NCBI)
Excitatory amino acid transporter 3
dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family
EAAC1excitatory amino acid carrier 1; EAAT3excitatory amino acid transporter 3; Excitatory amino-acid carrier 1; Neuronal and epithelial glutamate transporter; Sodium-dependent glutamate/aspartate transporter 3; solute carrier family 1 (neuronal/epithelial high affinity glutamatetransporter, system Xag), member 1; Solute carrier family 1 member 1 SLC1A1 DCBXA, EAAC1, EAAT3, SCZD18 solute carrier family 1 member 1 excitatory amino acid transporter 3|excitatory amino acid carrier 1|neuronal and epithelial glutamate transporter|sodium-dependent glutamate/aspartate transporter 3|solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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