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Product Info Summary
|Description:||Transient overexpression lysate of ER degradation enhancer, mannosidase alpha-like 3 (EDEM3)|
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EDEM3 (NM_025191) Human Over-expression Lysate
Transient overexpression lysate of ER degradation enhancer, mannosidase alpha-like 3 (EDEM3)
Storage & Handling
Cite This Product
EDEM3 (NM_025191) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS080267)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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Gene/Protein Information for EDEM3 (Source: Uniprot.org, NCBI)
ER degradation-enhancing alpha-mannosidase-like protein 3
glycosyl hydrolase 47 family
C1orf22; chromosome 1 open reading frame 22; ER degradation enhancer, mannosidase alpha-like 3; ER degradation-enhancing alpha-mannosidase-like 3; ER degradation-enhancing -mannosidase-like protein 3 EDEM3 C1orf22 ER degradation enhancing alpha-mannosidase like protein 3 ER degradation-enhancing alpha-mannosidase-like protein 3|ER degradation enhancer, mannosidase alpha-like 3|ER degradation-enhancing -mannosidase-like protein 3|ER degradation-enhancing alpha-mannosidase-like 3|alpha-1,2-mannosidase EDEM3*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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