Boster's Antibody Quality Control Panel

 

Boster Bio is proud to offer over 800 ELISA kits for a wide range of targets. With over 20 years of experience in antibody and ELISA kit manufacturing, Boster Picokine™ enzyme linked immunosorbent assay (ELISA) kits are guaranteed to be sensitive, specific, and stable. We rigorously validate every lot against a wide range of samples to ensure consistent, reliable results. Join over 14,000 scientists who put their trust in Boster Picokine™ ELISA kits.

Boster Picokine™ ELISA Kits are based on standard sandwich enzyme-linked immune-sorbent assay technology. A Capture antibody specific for the target analyte is precoated onto 96-well plates. The target analyte in test samples, standards, or controls is captured by this antibody. A biotinylated detection antibody that binds to a different epitope on the target analyte is added to the wells to complete the sandwich. Avidin-Biotin-Peroxidase Complex was added and HRP substrate TMB was used to produce a signal that is proportional to the amount of target analyte captured.

Benefits of Boster's Antibody Pairs

  • High Specificity
  • High Signal
  • Low background
  • Peak Sensitivity

At Boster Bio, we take great pride in the art of ELISA kits production. We have established a comprehensive ELISA development and validation system. Our technical resources are free to access and we share what we learn. Validation testing for all Boster ELISA Kits includes intra- and inter-assay precision, assay linearity, recovery, stability and specificity. We thoroughly validate every kit using biologically relevant matrices, including cell culture supernatant, serum, plasma, cell lysate, and tissue to ensure our kits will provide consistent results with any sample type.

Percision

Boster spends great efforts in documenting lot to lot variability and make sure our assay kits produce robust data that are reproducible. Coefficient of variation for intra and inter-assay precision are usually less than 10%.
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.

  Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean(pg/ml) 175 383 582 178 379 599
Standard Deviation 7.9 16.6 20.9 10.2 21.5 28.7
CV(%) 4.5 4.3 3.6 5.8 5.7 4.8

Stability

Boster ELISA kits are tested for stability. For instance, three samples with different concentrations of BDNF were assayed using the same lot of the Human BDNF ELISA kit over a 12 month period.

Linearity

Dilutions should always derive the same final analyte concentration for a sample. This is known as assay linearity. Bosterbio performs dilution linearity to demonstrate that a sample with a spiked concentration can be diluted to a concentration within the working range and still give a reliable result. We generate a dilution series using kit diluents across the dynamic range of the assay for each validated sample type. The results are expressed as a percent observed from expected. Values between 80–120% show good assay linearity.

Dilution Ratio Average (%) Range (%)
1:2 100 97-105
1:4 100 96-104
1:8 100 95-111
1:16 100 95-107

To assess the linearity of the assay, four serum samples from healthy human containing or spiked with high concentrations of human BDNF recombinant proteins were serially diluted to produce samples with values within the dynamic range of the assay.

Recovery

The spiking recovery test, a method to evaluate the accuracy, was analyzed by adding a known amount of analyte to the sample matrices, such as serum and plasma. Recovery was calculated by comparing the observed concentration as determined by ELISA and the expected concentration by summation. Low, medium, and high concentrations of analyte are spiked into all validated sample types and then analyzed for recovery. The results are expressed as a percentage of analyte recovered. Our acceptance range for the recovery is 80–120%.

Sample Average Recovery (%) Range (%)
Serum from healthy human 97 95-103
Plasma (heparin) from healthy human 95 92-102

Low, medium, and high concentrations of recombinant human BDNF were used as the spike in the spike and recovery experiments.

Specificity

Specificity was defined as the ability to selectively and precisely evaluate the target analyte regardless of the presence of other components in the testing sample. Picokine™ ELISA Kits are tested against related molecules to ensure no cross-reactivity or interference.